PPM1D Protein (AA 98-375) (His tag)
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- Target See all PPM1D Proteins
- PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 98-375
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PPM1D protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MVAFFAVCDG HGGREAAQFA REHFWGFIKK QKGFTSSEPA KVCAAIRKGF LACHLAMWKK LAEWPKTMTG LPSTSGTTAS VVIIRGMKMY VAHVGDSGVV LGIQDDPKDD FVRAVEVTQD HKPELPKERE RIEGLGGSVM NKSGVNRVVW KRPRLTHNGP VRRSTVIDQI PFLAVARALG DLWSYDFFSG EFVVSPEPDT SVHTLDPQKH KYIILGSDGL WNMIPPQDAI SMCQDQEEKK YLMGEHGQSC AKMLVNRALG RWRQRMLRAD NTSAIVICI
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product PPM1D Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid, In 20 mM Tris-HCl ( pH 8.0) containing 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))
- Alternative Name
- PPM1D (PPM1D Products)
- Synonyms
- PP2C-DELTA Protein, WIP1 Protein, AV338790 Protein, Wip1 Protein, zgc:103518 Protein, ppm1d Protein, wu:fj44c08 Protein, zgc:55836 Protein, zgc:77316 Protein, protein phosphatase, Mg2+/Mn2+ dependent 1D Protein, protein phosphatase 1D magnesium-dependent, delta isoform Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1D Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1Db Protein, protein phosphatase, Mg2+/Mn2+ dependent 1D L homeolog Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1Da Protein, PPM1D Protein, Ppm1d Protein, ppm1db Protein, ppm1d.L Protein, ppm1da Protein
- Background
- PPM1D also known as Protein phosphatase 1D magnesium-dependent, delta isoform. This protein is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase (MAPK/p38) through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. Recombinant human PPM1D, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 33.2kDa (299aa)
- Pathways
- p53 Signaling, Cell Division Cycle
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