VEGFC Protein (AA 47-413) (His tag)

Catalog No. ABIN6239783

Product Details

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Biological Activity: Active

Protein Characteristics: AA 47-413

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This VEGFC protein is labelled with His tag.

Application: Cell Culture (CC), Activity Assay (AcA)

Characteristics: Tag location: N-terminal His Tag

Purity: > 90 %

Biological Activity Comment: Vascular endothelial growth factor C (VEGFC) is a protein that is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It plays key roles in the physiology and pathology of many aspects of the cardiovascular system, including vasculogenesis, hematopoiesis, angiogenesis and vascular permeability. To test the effect of VEGFC on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. The dose-effect curve of VEGFC was shown in Figure 1. It was obvious that VEGFC significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 0.304 to 1.339μg/mL The dose-effect curve of VEGFC on ECV304 cells

Application Details

Application Notes: Isoelectric Point: 8.2

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Abstract: VEGFC Products

Synonyms: VEGFC Protein, vegf-c Protein, xvegf-c Protein, Flt4-L Protein, VRP Protein, AW228853 Protein, VEGF-C Protein, vascular endothelial growth factor c Protein, vascular endothelial growth factor C Protein, vascular endothelial growth factor C S homeolog Protein, vegfc Protein, VEGFC Protein, vegfc.S Protein, Vegfc Protein

Background: Alternative Names: VEGF-C, Flt4-L, VRPL, Vascular Endothelial Growth Factor-Related Protein

Molecular Weight: 45kDa

UniProt: P49767

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2