IL-5 Protein (AA 20-134) (His tag)
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- Target See all IL-5 (IL5) Proteins
- IL-5 (IL5) (Interleukin 5 (IL5))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 20-134
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This IL-5 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPIPTEIPT SALVKETLAL LSTHRTLLIA NETLRIPVPV HKNHQLCTEE IFQGIGTLES QTVQGGTVER LFKNLSLIKK YIDGQKKKCG EERRRVNQFL DYLQEFLGVM NTEWIIESHH HHHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 1.5 ng/ml.
- Top Product
- Discover our top product IL5 Protein
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Interleukin 5 (IL5) (AA 20-134) protein (His-Avi Tag)
IL5 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 20-134) protein (His-Avi Tag,Biotin)IL5 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 21-133) protein (His-Avi Tag)IL5 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 20-134) protein (His tag)IL5 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 21-133) protein (His-Avi Tag,Biotin)IL5 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 20-132) protein (His tag)IL5 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 5 (IL5) (AA 21-133) protein (His tag)IL5 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 5 (IL5) (AA 1-134) protein (GST tag)IL5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 5 (IL5) (AA 20-134) protein (GST tag)IL5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 5 (IL5) protein (His tag)IL5 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- IL-5 (IL5) (Interleukin 5 (IL5))
- Alternative Name
- IL5 (IL5 Products)
- Synonyms
- EDF Protein, IL-5 Protein, TRF Protein, Il-5 Protein, IL5 Protein, interleukin 5 Protein, IL5 Protein, Il5 Protein
- Background
- IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. It related hematopoietic cytokines that are important for allergic inflammation. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. Recombinant human IL5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 14.2kDa (124aa) 13.5-18kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_000870
- UniProt
- P05113
- Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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