SERPIND1 Protein (His tag)
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- Target See all SERPIND1 Proteins
- SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SERPIND1 protein is labelled with His tag.
- Purpose
- Recombinant Human SerpinD1 Protein (His Tag)(Active)
- Sequence
- Met 1-Ser 499
- Characteristics
- A DNA sequence encoding the human SerpinD1 precursor (NP_000176.2) (Met 1-Ser 499) was expressed with a C-terminal polyhistidine tag.
- Purity
- > 97 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized recombinant human SerpinD1-His at 10 μg/ml (100 μl/well) can bind biotinylated recombinant mouse ELANE-His with a linear range of 0.15-10.0 μg/ml.
- Top Product
- Discover our top product SERPIND1 Protein
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serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) (AA 1-499) protein (GST tag)
SERPIND1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) protein (His tag)SERPIND1 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) (Active) protein (His tag)SERPIND1 Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))
- Alternative Name
- SerpinD1 (SERPIND1 Products)
- Synonyms
- D22S673 Protein, HC2 Protein, HCF2 Protein, HCII Protein, HLS2 Protein, LS2 Protein, THPH10 Protein, serpind1 Protein, MGC53936 Protein, rls2var1 Protein, AA985900 Protein, AI303446 Protein, Hcf2 Protein, serpin family D member 1 Protein, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 L homeolog Protein, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 Protein, serine (or cysteine) peptidase inhibitor, clade D, member 1 Protein, SERPIND1 Protein, serpind1.L Protein, Serpind1 Protein, serpind1 Protein
- Background
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Background: SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Synonym: D22S673;HC2;HCF2;HCII;HLS2;LS2;SerpinD1;THPH10
- Molecular Weight
- 56.4 kDa
- NCBI Accession
- NP_000176
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