SIGLEC15 Protein (AA 60-322) (His-Avi Tag)
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- Target See all SIGLEC15 Proteins
- SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 60-322
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Origin
- Cynomolgus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SIGLEC15 protein is labelled with His-Avi Tag.
- Purpose
- Cynomolgus Siglec-15/CD33L3 Protein
- Sequence
- Met60-Thr322
- Characteristics
- Recombinant Cynomolgus Siglec-15/CD33L3 Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Met60-Thr322.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus Siglec-15, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as 0.22μm filtered solution in 20 mM PB, 500 mM NaCl, 0.1M L-arginine ( pH 6.0).
- Storage
- -80 °C
- Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))
- Alternative Name
- Siglec-15 (SIGLEC15 Products)
- Synonyms
- RGD1565948 Protein, CD33L3 Protein, HsT1361 Protein, SIGLEC-15 Protein, Cd33l3 Protein, EG620235 Protein, SIGLEC-I Protein, sialic acid binding Ig-like lectin 15 Protein, sialic acid binding Ig like lectin 15 Protein, Siglec15 Protein, SIGLEC15 Protein
- Background
- Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
- Molecular Weight
- 29.2 kDa. Due to glycosylation, the protein migratesto 33-43 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- XP_005586866
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