SIGLEC15 Protein (AA 60-322) (His-Avi Tag,Biotin)

Catalog No. ABIN7275585

Product Details

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Protein Type: Recombinant

Protein Characteristics: AA 60-322

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This SIGLEC15 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Cynomolgus Siglec-15/CD33L3 Protein

Sequence: Met60-Thr322

Characteristics: Recombinant Biotinylated Cynomolgus Siglec-15/CD33L3 Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Met60-Thr322.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Human Siglec-15 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus Siglec-15, His Tag with the EC50 of 23.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 20 mM PB, 500 mM NaCl, 0.1M L-arginine ( pH 6.0).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Alternative Name: Siglec-15 (SIGLEC15 Products)

Synonyms: RGD1565948 Protein, CD33L3 Protein, HsT1361 Protein, SIGLEC-15 Protein, Cd33l3 Protein, EG620235 Protein, SIGLEC-I Protein, sialic acid binding Ig-like lectin 15 Protein, sialic acid binding Ig like lectin 15 Protein, Siglec15 Protein, SIGLEC15 Protein

Background: Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.

Molecular Weight: 29.2 kDa. Due to glycosylation, the protein migratesto 33-43 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005586866