SIGLEC15 Protein (AA 20-263) (His-Avi Tag)

Catalog No. ABIN7275607

Product Details

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Protein Type: Recombinant

Protein Characteristics: AA 20-263

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SIGLEC15 protein is labelled with His-Avi Tag.

Purpose: Human Siglec-15/CD33L3 Protein

Sequence: Phe20-Thr263

Characteristics: Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe20-Thr263.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAC ( pH 5.0).

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM NaAC ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Alternative Name: Siglec-15 (SIGLEC15 Products)

Synonyms: RGD1565948 Protein, CD33L3 Protein, HsT1361 Protein, SIGLEC-15 Protein, Cd33l3 Protein, EG620235 Protein, SIGLEC-I Protein, sialic acid binding Ig-like lectin 15 Protein, sialic acid binding Ig like lectin 15 Protein, Siglec15 Protein, SIGLEC15 Protein

Background: Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates..

Molecular Weight: 29.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.

UniProt: Q6ZMC9