SIGLEC15 Protein (AA 24-260) (Fc Tag)
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- Target See all SIGLEC15 Proteins
- SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-260
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SIGLEC15 protein is labelled with Fc Tag.
- Purpose
- Human Siglec-15/CD33L3 Protein
- Sequence
- Lys24-Gly260
- Characteristics
- Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys24-Gly260.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Siglec-15, hFc Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-Siglec-15 Ab., hFc Tag with the EC50 of 7.37ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product SIGLEC15 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAC ( pH 5.0).
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM NaAC ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))
- Alternative Name
- Siglec-15 (SIGLEC15 Products)
- Synonyms
- RGD1565948 Protein, CD33L3 Protein, HsT1361 Protein, SIGLEC-15 Protein, Cd33l3 Protein, EG620235 Protein, SIGLEC-I Protein, sialic acid binding Ig-like lectin 15 Protein, sialic acid binding Ig like lectin 15 Protein, Siglec15 Protein, SIGLEC15 Protein
- Background
- Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
- Molecular Weight
- 52.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
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