FAP Protein (His tag)
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- Target See all FAP Proteins
- FAP (Fibroblast Activation Protein, alpha (FAP))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FAP protein is labelled with His tag.
- Sequence
- Leu26-Asp760
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FAP, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product FAP Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 250 mM NaCl ( pH 8.2). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- FAP (Fibroblast Activation Protein, alpha (FAP))
- Alternative Name
- FAP (FAP Products)
- Synonyms
- fap-A Protein, gene 13 Protein, FAP Protein, fapa Protein, dppiv Protein, seprase Protein, DPPIV Protein, FAPA Protein, fibroblast activation protein Protein, fibroblast activation protein alpha L homeolog Protein, fibroblast activation protein alpha Protein, fibroblast activation protein, alpha Protein, prolyl endopeptidase FAP Protein, Fap Protein, fap.L Protein, FAP Protein, fap Protein, LOC100547273 Protein
- Background
- FAP, FAPalpha, SIMP, Seprase, APCE, DPPIV, DPPIVA, FAPA,Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
- Molecular Weight
- 86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
- Pathways
- Tube Formation
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