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FAP Protein (His tag)

FAP Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Catalog No. ABIN7274583
  • Target See all FAP Proteins
    FAP (Fibroblast Activation Protein, alpha (FAP))
    Protein Type
    Recombinant
    Origin
    • 17
    • 10
    • 3
    • 3
    Human
    Source
    • 17
    • 6
    • 4
    • 2
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This FAP protein is labelled with His tag.
    Sequence
    Leu26-Asp760
    Purity
    > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human FAP, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA. See testing image for detail.
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    Discover our top product FAP Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in 20 mM Tris, 250 mM NaCl ( pH 8.2). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    4 °C,-80 °C
    Storage Comment
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    FAP (Fibroblast Activation Protein, alpha (FAP))
    Alternative Name
    FAP (FAP Products)
    Synonyms
    fap-A Protein, gene 13 Protein, FAP Protein, fapa Protein, dppiv Protein, seprase Protein, DPPIV Protein, FAPA Protein, fibroblast activation protein Protein, fibroblast activation protein alpha L homeolog Protein, fibroblast activation protein alpha Protein, fibroblast activation protein, alpha Protein, prolyl endopeptidase FAP Protein, Fap Protein, fap.L Protein, FAP Protein, fap Protein, LOC100547273 Protein
    Background
    FAP, FAPalpha, SIMP, Seprase, APCE, DPPIV, DPPIVA, FAPA,Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
    Molecular Weight
    86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
    Pathways
    Tube Formation
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