FAP Protein (AA 26-760) (Fc Tag)

Catalog No. ABIN7274584

Product Details

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Protein Type: Recombinant

Protein Characteristics: AA 26-760

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FAP protein is labelled with Fc Tag.

Purpose: Human FAP Protein

Sequence: Leu26-Asp760

Characteristics: Recombinant Human FAP Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Leu26-Asp760.

Purity: > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FAP, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 33.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAP (FAP Products)

Synonyms: fap-A Protein, gene 13 Protein, FAP Protein, fapa Protein, dppiv Protein, seprase Protein, DPPIV Protein, FAPA Protein, fibroblast activation protein Protein, fibroblast activation protein alpha L homeolog Protein, fibroblast activation protein alpha Protein, fibroblast activation protein, alpha Protein, prolyl endopeptidase FAP Protein, Fap Protein, fap.L Protein, FAP Protein, fap Protein, LOC100547273 Protein

Background: Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.

Molecular Weight: 112.3 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.

Pathways: Tube Formation