GUCY2C Protein (AA 24-430) (His tag)

Catalog No. ABIN7274713

Product Details

Target: GUCY2C (Guanylate Cyclase 2C (Heat Stable Enterotoxin Receptor) (GUCY2C))

Protein Type: Recombinant

Protein Characteristics: AA 24-430

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This GUCY2C protein is labelled with His tag.

Purpose: Cynomolgus GUCY2C/Guanylyl cyclase C Protein

Sequence: Ser24-Gln430

Characteristics: Recombinant Cynomolgus GUCY2C/Guanylyl cyclase C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser24-Gln430.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus GUCY2C, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GUCY2C Antibody, hFc Tag with the EC50 of 103.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GUCY2C (Guanylate Cyclase 2C (Heat Stable Enterotoxin Receptor) (GUCY2C))

Alternative Name: GUCY2C (GUCY2C Products)

Synonyms: DIAR6 Protein, GUC2C Protein, MECIL Protein, MUCIL Protein, STAR Protein, AI893437 Protein, GC-C Protein, Gcc Protein, GCC Protein, gucy2c-A Protein, GUCY2C Protein, Gucy2c Protein, guanylate cyclase 2C Protein, guanylate cyclase 2c Protein, guanylate cyclase 2C L homeolog Protein, GUCY2C Protein, Gucy2c Protein, gucy2c.L Protein, gucy2c Protein

Background: Guanylyl cyclase C (GUCY2C) has canonical centrality in defense of key intestinal homeostatic mechanisms, encompassing fluid and electrolyte balance, epithelial dynamics, antitumorigenesis, and intestinal barrier function. GUCY2C may represent a new target for anti-obesity pharmacotherapy.

Molecular Weight: 47.2 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005570270