GDF15 Protein (AA 197-308) (Fc Tag,Biotin)

Catalog No. ABIN7274726

Product Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Protein Characteristics: AA 197-308

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This GDF15 protein is labelled with Fc Tag,Biotin.

Purpose: Biotinylated Human GDF15 Protein (Primary Amine Labeling)

Sequence: Ala197-Ile308

Characteristics: Recombinant Biotinylated Human GDF15 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala197-Ile308.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15 (GDF15 Products)

Synonyms: GDF15 Protein, GDF-15 Protein, MIC-1 Protein, MIC1 Protein, NAG-1 Protein, PDF Protein, PLAB Protein, PTGFB Protein, SBF Protein, growth differentiation factor 15 Protein, GDF15 Protein, Gdf15 Protein

Background: Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.

Molecular Weight: 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome