ErbB2/Her2 Protein (AA 23-652) (His tag)

Catalog No. ABIN7274794

Product Details

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Protein Type: Recombinant

Protein Characteristics: AA 23-652

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This ErbB2/Her2 protein is labelled with His tag.

Purpose: Cynomolgus Her2/ErbB2 Protein

Sequence: Thr23-Thr652

Characteristics: Recombinant Cynomolgus Her2/ErbB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr23-Thr652.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus Her2, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: Her2 (ErbB2/Her2 Products)

Synonyms: CD340 Protein, HER-2 Protein, HER-2/neu Protein, HER2 Protein, MLN 19 Protein, NEU Protein, NGL Protein, TKR1 Protein, Erbb-2 Protein, Neu Protein, c-erbB2 Protein, c-neu Protein, mKIAA3023 Protein, wu:fv70f10 Protein, zgc:63601 Protein, erb2 Protein, erb-b2 receptor tyrosine kinase 2 Protein, ERBB2 Protein, Erbb2 Protein, erbb2 Protein

Background: ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.

Molecular Weight: 70.4 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005584091

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development