ErbB2/Her2 Protein (AA 489-630) (His-Avi Tag,Biotin)

Catalog No. ABIN7274804

Product Details

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Protein Type: Recombinant

Protein Characteristics: AA 489-630

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ErbB2/Her2 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human Her2/ErbB2 Domain 4 Protein

Sequence: Pro489-Cys630

Characteristics: Recombinant Biotinylated Human Her2/ErbB2 Domain 4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro489-Cys630.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: Her2 (ErbB2/Her2 Products)

Synonyms: CD340 Protein, HER-2 Protein, HER-2/neu Protein, HER2 Protein, MLN 19 Protein, NEU Protein, NGL Protein, TKR1 Protein, Erbb-2 Protein, Neu Protein, c-erbB2 Protein, c-neu Protein, mKIAA3023 Protein, wu:fv70f10 Protein, zgc:63601 Protein, erb2 Protein, erb-b2 receptor tyrosine kinase 2 Protein, ERBB2 Protein, Erbb2 Protein, erbb2 Protein

Background: ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane.

Molecular Weight: 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development