CD51/CD61 Protein (AA 31-992) (His-Avi Tag)

Catalog No. ABIN7275059

Product Details

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Protein Type: Recombinant

Protein Characteristics: AA 31-992

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD51/CD61 protein is labelled with His-Avi Tag.

Purpose: Human Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer Protein

Sequence: Phe31-Val992 (ITGAV) acidic tail & Gly27-Asp718 (ITGB3) basic tail

Specificity: Uni-Prot: P06756-1 (ITGAV), P05106-1 (ITGB3)

Characteristics: Recombinant Human Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992(ITGAV) acidic tail & GLy27-Asp718(ITGB3) basic tail.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ITGAV&ITGB3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Alternative Name: Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer (ITGAV/ITGB3 Products)

Synonyms: CD51 Protein, MSK8 Protein, VNRA Protein, VTNR Protein, integrin subunit alpha V Protein, ITGAV Protein

Background: ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling.

Molecular Weight: 114 kDa (ITGAV)&81.3 kDa (ITGB3). Due to glycosylation,the protein migrates to 85-140 kDa based on Tris-Bis PAGE result.