IL12B Protein (AA 23-328) (His tag)
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- Target See all IL12B Proteins
- IL12B (Interleukin 12 beta (IL12B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-328
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL12B protein is labelled with His tag.
- Purpose
- Human IL-12B/p40/NKSF2 Protein
- Sequence
- Ile23-Ser328
- Characteristics
- Recombinant Human IL-12B/p40/NKSF2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Ser328.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-12B, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL12B (Interleukin 12 beta (IL12B))
- Alternative Name
- IL-12B (IL12B Products)
- Synonyms
- CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, IL-12 p40 Protein, il12b Protein, il12p40.b Protein, zgc:152789 Protein, IL-12.p40 Protein, IL12p40 Protein, IL12B Protein, CLMF p40 Protein, interleukin 12B Protein, interleukin 12b Protein, interleukin 12Ba Protein, IL12B Protein, Il12b Protein, il12ba Protein
- Background
- Interleukin (IL)-12B, which encodes the p40 subunit common to IL-12 and IL-23, as one of the genes for which expression in fibroblast-like synoviocytes from patients with rheumatoid arthritis (RA-FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin-related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor-like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.
- Molecular Weight
- 34.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
- UniProt
- P29460
- Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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