GM-CSF Protein (His tag)
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- Target See all GM-CSF (CSF2) Proteins
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Rat
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GM-CSF protein is labelled with His tag.
- Purpose
- Active Recombinant Rat CSF-2/GM-CSF Protein
- Sequence
- APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK
- Specificity
- Ala18-Lys144
- Purity
- > 97 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 1.0 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured in a cell proliferation assay using SP2/O mouse myeloma cells. The ED50 for this effect is 7.5-30 pg/mL.
- Top Product
- Discover our top product CSF2 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- Alternative Name
- CSF-2/GM-CSF (CSF2 Products)
- Synonyms
- GMCSF Protein, GM-CSF Protein, CSF2 Protein, Csfgm Protein, Gm-CSf Protein, MGI-IGM Protein, Gm-csf Protein, Gmcsf Protein, CSF Protein, colony stimulating factor 2 Protein, colony stimulating factor 2 (granulocyte-macrophage) Protein, CSF2 Protein, Csf2 Protein
- Background
- GMCSF,CSF2
- Gene ID
- 116630
- UniProt
- P48750
- Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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