FLT1 Protein (His tag)
-
- Target See all FLT1 Proteins
- FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
- Protein Type
- Recombinant
- Biological Activity
- Active
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FLT1 protein is labelled with His tag.
- Purpose
- Active Recombinant Human FLT-1/VEGFR-1 Protein
- Sequence
- MVSYWDTGVL LCALLSCLLL TGSSSGSKLK DPELSLKGTQ HIMQAGQTLH LQCRGEAAHK WSLPEMVSKE SERLSITKSA CGRNGKQFCS TLTLNTAQAN HTGFYSCKYL AVPTSKKKET ESAIYIFISD TGRPFVEMYS EIPEIIHMTE GRELVIPCRV TSPNITVTLK KFPLDTLIPD GKRIIWDSRK GFIISNATYK EIGLLTCEAT VNGHLYKTNY LTHRQTNTII DVQISTPRPV KLLRGHTLVL NCTATTPLNT RVQMTWSYPD EKNKRASVRR RIDQSNSHAN IFYSVLTIDK MQNKDKGLYT CRVRSGPSFK SVNTSVHIYD KAFITVKHRK QQVLETVAGK RSYRLSMKVK AFPSPEVVWL KDGLPATEKS ARYLTRGYSL IIKDVTEEDA GNYTILLSIK QSNVFKNLTA TLIVNVKPQI YEKAVSSFPD PALYPLGSRQ ILTCTAYGIP QPTIKWFWHP CNHNHSEARC DFCSNNEESF ILDADSNMGN RIESITQRMA IIEGKNKMAS TLVVADSRIS GIYICIASNK VGTVGRNISF YITDVPNGFH VNLEKMPTEG EDLKLSCTVN KFLYRDVTWI LLRTVNNRTM HYSISKQKMA ITKEHSITLN LTIMNVSLQD SGTYACRARN VYTGEEILQK KEITIRDQEA PYLLRNLSDH TVAISSSTTL DCHANGVPEP QITWFKNNHK IQQEPGIILG PGSSTLFIER VTEEDEGVYH CKATNQKGSV ESSAYLTVQG TSDKSN
- Specificity
- Met1-Asn756
- Purity
- > 95 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGFR1 at 500 ng/mL (100 μL/well) can bind Recombinant Mouse PLGF with a linear range of 12-49 ng/mL.|2.Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.039-0.154 ng/mL.
- Top Product
- Discover our top product FLT1 Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
-
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
-
- Target
- FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
- Alternative Name
- FLT-1/VEGFR-1 (FLT1 Products)
- Synonyms
- FLT Protein, FLT-1 Protein, VEGFR-1 Protein, VEGFR1 Protein, FLT1 Protein, flt1b Protein, sFlt1 Protein, AI323757 Protein, Flt-1 Protein, fms related tyrosine kinase 1 Protein, FMS-related tyrosine kinase 1 Protein, vascular endothelial growth factor receptor 1 Protein, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) Protein, FMS-like tyrosine kinase 1 Protein, vascular endothelial growth factor receptor-1 Protein, FLT1 Protein, Flt1 Protein, CpipJ_CPIJ012087 Protein, flt1 Protein, FLT-1 Protein
- Background
- FLT,FLT-1,VEGFR-1,VEGFR1,FLT1
- Gene ID
- 2321
- UniProt
- P17948
- Pathways
- RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
-