TNF alpha Protein (AA 77-233) (His tag)

Catalog No. ABIN2018406

Product Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 77-233

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TNF alpha protein is labelled with His tag.

Characteristics: ED50<0.13 ng/mL, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >7.69 x 10^6 units/mg.
AA 77-233, expressed with an N-terminal Met and 6×His.

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 1.0 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhTNF-alpha should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

Expiry Date: 12 months

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha (TNF alpha Products)

Synonyms: DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein

Background: Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) produced in E. coli is a non-glycosylated polypeptide chain of 164 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 18.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Synonyms: Tumor Necrosis Factor-alpha (TNF-alpha), Cachectin.

Molecular Weight: 18.3kDa, observed by reducing SDS-PAGE.

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome