anti-Human Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic antibody for Immunofluorescence

Recommended Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic Antibody

Antigen
Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) Antibodies
  • GAPDS
  • GAPDHS
  • GAPD2
  • GAPDH-2
  • HSD-35
  • Gapd-s
  • Gapds
  • gapdh-2
  • cb350
  • fb71f08
  • fk58c09
  • g3pdh
  • gapdh
  • gapds
  • wu:fb71f08
  • wu:fk58c09
  • zgc:76908
  • glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
  • GAPDHS
  • Gapdhs
  • gapdhs
Reactivity
Human, Pig (Porcine)
66
30
28
6
4
2
2
2
1
Host
Mouse
53
12
2
Clonality
Monoclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
48
13
11
10
8
7
4
3
1
1
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Available images

Catalog No. ABIN269088
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General

Antigen Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) Antibodies
Reactivity Human, Pig (Porcine)
(66), (30), (28), (6), (4), (2), (2), (2), (1)
Host Mouse
(53), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(48), (13), (11), (10), (8), (7), (4), (3), (1), (1)
Supplier

Product details

Target Details Application Details Handling Images
Specificity The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, previously identified under the general name "intra-acrosomal protein".
Purification Size Exclusion Chromatography
Immunogen Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Clone Hs-8
Isotype IgM

Target Details

Product details Application Details Handling Images back to top
Antigen
Alternative Name GAPDH-2 (GAPDHS Antibody Abstract)
Background Gene Symbol: GAPDHS
Gene ID 26330
Pathways Regulation of Carbohydrate Metabolic Process

Application Details

Product details Target Details Handling Images back to top
Application Notes Western Blot 1:1000, Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000Western Blot Detects bands of approximately 220, 50, 34 and 21 kDa. Immunocytochemistry: Staining technique: Membrane permeabilization (acetone) is essential.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target Details Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer TBS (approx. pH 8.0)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

Images

Product details Target Details Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibody (ABIN269088) Flow Cytometry: GAPDH-2 Antibody (Hs-8) - Detection of GAPDHS in acrosomes of human ...