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|Antigen||REST Corepressor 1 (RCOR1) Antibodies|
|Epitope||AA 78-192 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CoREST antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-CoREST AntibodyTarget Details CoREST Application Details Handling References for anti-CoREST antibody (ABIN968756) Images
|Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human CoREST aa. 78-192|
Target Details CoRESTProduct Details anti-CoREST Antibody Application Details Handling References for anti-CoREST antibody (ABIN968756) Images back to top
|Alternative Name||CoRest (RCOR1 Antibody Abstract)|
|Background||Histone deacetylases (HDACs) are found in large, multisubunit complexes. HDAC1 and HDAC2 are found predominately in either an mSin3A-containing complex or a complex containing the ATP-dependent chromatin remodeling protein, CHD4. The mSin3A complex is recrutied by DNA-binding transcription factors, such as the unligand hormone receptors. In these situations, the HDAC/mSin3A complex is thought to be involved in gene repression. CoREST is a corepressor for REST (RE1 silencing transcription factor) that is found in complex with mSin3A, as well as in non-mSin3A HDAC complexes. CoREST contains an N-terminal and a C-terminal SANT domain, which is common to corepressors involved in steroid horomone receptor gene repression. In non-neuronal cells, CoREST and REST mediate repression of the type II sodium channel promoter. REST is usually found in complex with mSin3A, and under certain conditions may recruit CoREST for repressor activity. The N-terminal SANT domain of CoREST is required for interaction with HDAC1 to form an HDAC complex that does not contain mSin3A or REST. Thus, CoREST is a corepressor that may interact with distinct protein complexes depending on the target for gene repression. This antibody is routinely tested by western blot analysis.|
|Molecular Weight||66 kDa|
|Pathways||Regulation of Hormone Metabolic Process, Chromatin Binding|
Application DetailsProduct Details anti-CoREST Antibody Target Details CoREST Handling References for anti-CoREST antibody (ABIN968756) Images back to top
Related Products: ABIN968537, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-CoREST Antibody Target Details CoREST Application Details References for anti-CoREST antibody (ABIN968756) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-CoREST antibody (ABIN968756)Product Details anti-CoREST Antibody Target Details CoREST Application Details Handling Images back to top
|Product cited in:||
You, Tong, Grozinger, Schreiber: "CoREST is an integral component of the CoREST- human histone deacetylase complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 4, pp. 1454-8, 2001
Grimes, Nielsen, Battaglioli, Miska, Speh, Berry, Atouf, Holdener, Mandel, Kouzarides: "The co-repressor mSin3A is a functional component of the REST-CoREST repressor complex." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9461-7, 2000
Andrés, Burger, Peral-Rubio, Battaglioli, Anderson, Grimes, Dallman, Ballas, Mandel: "CoREST: a functional corepressor required for regulation of neural-specific gene expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9873-8, 1999
ImagesProduct Details anti-CoREST Antibody Target Details CoREST Application Details Handling References for anti-CoREST antibody (ABIN968756) back to top