anti-Human Argininosuccinate Lyase antibody for Immunofluorescence (Cultured Cells)

Recommended Argininosuccinate Lyase Antibody (supplied by: Log in to see )

Antigen
Argininosuccinate Lyase (ASL) Antibodies
  • ASAL
  • 2510006M18Rik
  • zgc:63532
  • BA4879
  • PSPTO0125
  • argininosuccinate lyase
  • argininosuccinate lyase ArgH
  • argininosuccinate lyase L homeolog
  • ASL
  • Asl
  • asl
  • argH2
  • argH
  • arg7
  • CNC04420
  • STHERM_c13370
  • asl.L
Epitope
AA 86-325
15
4
4
2
2
2
1
1
1
1
1
1
Reactivity
Human
66
39
30
6
6
6
6
6
5
4
3
3
3
3
3
3
2
Host
Rabbit
55
11
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
39
14
13
13
9
9
7
4
2
2
2
1
Options
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Catalog No. ABIN2800354
$ 431.20
Plus shipping costs $45.00

General

Antigen Argininosuccinate Lyase (ASL) Antibodies
Epitope AA 86-325
(15), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(66), (39), (30), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (2)
Host Rabbit
(55), (11)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(39), (14), (13), (13), (9), (9), (7), (4), (2), (2), (2), (1)
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Product details

Target Details Application Details Handling Images
Immunogen Recombinant protein fragment contain a sequence corresponding to a region within amino acids 86-325 of ASL
Isotype IgG

Target Details

Product details Application Details Handling Images back to top
Antigen
Alternative Name ASL (ASL Antibody Abstract)
Background Synonyms: ASAL antibody, ASL antibody, arginosuccinase antibody, argininosuccinase antibody, argininosuccinate lyase antibody
Molecular Weight 52 kDa
Pathways Response to Growth Hormone Stimulus

Application Details

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Application Notes Tested ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer ( pH 6.0) or Tris-EDTA buffer ( pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.ASL antibody detects ASL protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4 % paraformaldehyde at RT for 15 min.Green: ASL protein stained by ASL antibody (107-10865) diluted at 1:500.Blue: Hoechst 33342 staining.Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using ASL(107-10865) antibody at 1:500 dilution.Sample (30 μg of whole cell lysate)A: MOLT4 10 % SDS PAGE107-10865 diluted at 1:1000
Comment

ASL antibody, 1 mg/ml (100 μl)

Restrictions For Research Use only

Handling

Product details Target Details Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.