anti-Human GRB2 antibody for ELISA

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • grb2b
Epitope
C-Term
33
18
14
8
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
169
66
65
15
10
8
7
5
5
4
3
3
3
3
1
1
1
Host
Goat
120
25
23
2
Clonality
Polyclonal
Conjugate
This GRB2 antibody is un-conjugated
9
5
4
3
3
3
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
155
81
74
27
14
6
5
5
4
1
1
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Catalog No. ABIN184670
$ 429.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16 ABIN5901224 ELISA WB Goat Log in to see Polyclonal 0
15.755681 ABIN3184932 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
13 ABIN2562931 IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
12.755681 ABIN4901620 ELISA WB Mouse IgG1 Log in to see 5G3-E7-F11 0
12.755681 ABIN5539730 ELISA WB Biotin Goat C-Term Log in to see Polyclonal 0
12.755681 ABIN5617913 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
12.755681 ABIN2141186 IHC ELISA WB Rabbit IgG Ser159 Log in to see Polyclonal 0
12.755681 ABIN2140714 ELISA WB Rabbit IgG pSer159 Log in to see Polyclonal 0
12.755681 ABIN2142852 ELISA WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN6262078 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN3031114 IHC ELISA WB Rabbit Ig Fraction AA 89-118 Log in to see Polyclonal 0
7 ABIN1532105 IHC ELISA WB Rabbit IgG AA 125-174, pSer159 Log in to see Polyclonal 2
7 ABIN481756 ELISA IHC IHC (p) WB Goat AA 206-217 Log in to see Polyclonal 0
7 ABIN2465225 IHC ELISA WB Goat C-Term Log in to see Polyclonal 0
7 ABIN3031112 IHC ELISA WB Rabbit Ig Fraction AA 187-216 Log in to see Polyclonal 0
7 ABIN516177 ELISA WB Mouse IgM kappa AA 1-217, full length Log in to see 4C6-H6 0
4 ABIN1534949 ELISA WB Rabbit IgG AA 141-190 Log in to see Polyclonal 2
4 ABIN6271590 ELISA WB Rabbit IgG pTyr614 Log in to see Polyclonal 0
4 ABIN2890352 ELISA WB Rabbit IgG AA 141-190 Log in to see Polyclonal 0
4 ABIN6258224 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope C-Term
(33), (18), (14), (8), (6), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(169), (66), (65), (15), (10), (8), (7), (5), (5), (4), (3), (3), (3), (3), (1), (1), (1)
Host Goat
(120), (25), (23), (2)
Clonality
Polyclonal
Conjugate This GRB2 antibody is un-conjugated
(9), (5), (4), (3), (3), (3)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(155), (81), (74), (27), (14), (6), (5), (5), (4), (1), (1)
Pubmed 1 reference available
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling References for anti-GRB2 antibody (ABIN184670) Images
Purpose GRB2
Blocking Peptide Blocking peptide for this product available: ABIN369579
Sequence PRNYVTPVNR NV
Specificity This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Predicted Reactivity Human, Mouse, Rat, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.
Plasmids, Primers & others

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling References for anti-GRB2 antibody (ABIN184670) Images back to top
Antigen
Alternative Name GRB2 (GRB2 Antibody Abstract)
Background GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, abundant SRC homology
Gene ID 2885
NCBI Accession NP_002077, NP_987102
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling References for anti-GRB2 antibody (ABIN184670) Images back to top
Application Notes

DS WB Results: Approx 26 kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2 kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use at 0.05-0.15 μg/mL.

DS IHC Results: In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details References for anti-GRB2 antibody (ABIN184670) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-GRB2 antibody (ABIN184670)

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling Images back to top
Background publications

Lowenstein, Daly, Batzer, Li, Margolis, Lammers, Ullrich, Skolnik, Bar-Sagi, Schlessinger: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling References for anti-GRB2 antibody (ABIN184670) back to top
Supplier Images
Western Blotting (WB) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN184670) ABIN184670 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per la...
Immunohistochemistry (IHC) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN184670) ABIN184670 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen ...