anti-Human GRB2 antibody for Western Blotting

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • grb2b
Epitope
C-Term
33
16
14
8
6
5
5
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
170
67
66
16
11
9
7
6
5
4
3
3
3
3
1
1
1
Host
Rabbit
118
27
25
2
Clonality
Polyclonal
Conjugate
This GRB2 antibody is un-conjugated
9
5
4
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
156
84
75
29
14
5
5
5
4
1
1
Options
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Catalog No. ABIN2854697
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
20.056355 ABIN184670 IHC ELISA WB Goat C-Term Log in to see Polyclonal 1
20.056355 ABIN2854311 IHC (p) WB Rabbit IgG full length Log in to see Polyclonal 0
20.056355 ABIN6141378 IF IHC WB Rabbit Log in to see Polyclonal 0
17.056355 ABIN3184932 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
17.056355 ABIN302091 WB Rabbit IgG C-Term, AA 198-217 Log in to see Polyclonal 2
17.056355 ABIN5663552 WB Rabbit Log in to see Polyclonal 0
16.803753 ABIN5901224 ELISA WB Goat Log in to see Polyclonal 0
13.803753 ABIN2787701 WB Rabbit N-Term Log in to see Polyclonal 0
13.803753 ABIN2562931 IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
13.252602 ABIN4901620 ELISA WB Mouse IgG1 Log in to see 5G3-E7-F11 0
13.252602 ABIN5539730 ELISA WB Biotin Goat C-Term Log in to see Polyclonal 0
13.252602 ABIN5617913 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
13.252602 ABIN2141186 IHC ELISA WB Rabbit IgG Ser159 Log in to see Polyclonal 0
13.252602 ABIN2142852 ELISA WB Rabbit IgG Log in to see Polyclonal 0
13.252602 ABIN2140276 WB Rabbit IgG C-Term Log in to see Polyclonal 0
13.252602 ABIN2140714 ELISA WB Rabbit IgG pSer159 Log in to see Polyclonal 0
13 ABIN574433 IHC IHC (p) WB Rabbit AA 198-217, C-Term Log in to see Polyclonal 0
12.303753 ABIN390236 IHC (p) WB Rabbit Ig Fraction AA 187-216, Tyr209 Log in to see Polyclonal 3
12.303753 ABIN545861 IHC (p) ELISA WB Rabbit Log in to see Polyclonal 4
12.283474 ABIN257708 ELISA IHC IHC (p) WB Goat C-Term, both Isoforms Log in to see Polyclonal 1

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope C-Term
(33), (16), (14), (8), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(170), (67), (66), (16), (11), (9), (7), (6), (5), (4), (3), (3), (3), (3), (1), (1), (1)
Host Rabbit
(118), (27), (25), (2)
Clonality
Polyclonal
Conjugate This GRB2 antibody is un-conjugated
(9), (5), (4), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(156), (84), (75), (29), (14), (5), (5), (5), (4), (1), (1)
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling Images
Cross-Reactivity Rat (Rattus), Xenopus tropicalis, Pig (Porcine), Chicken, Chimpanzee, Cow (Bovine)
Cross-Reactivity (Details) Rat (100 %), Xenopus Tropicalis (92 %), Pig (100 %), Chicken (92 %), Chimpanzee (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to GRB2 (growth factor receptor-bound protein 2)
GRB2 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRB2. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GRB2 (GRB2 Antibody Abstract)
Background The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Golgi apparatus
Molecular Weight 25 kDa
Gene ID 2885
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 , JC , BCL-1

Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN2854697) WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 12% SDS PAGE antibod...
Immunohistochemistry (IHC) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN2854697) IHC-P Image GRB2 antibody detects GRB2 protein at nucleus on mouse liver by immunohis...
Immunohistochemistry (IHC) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN2854697) IHC-P Image GRB2 antibody detects GRB2 protein at nucleus on rat fore brain by immuno...
Immunohistochemistry (IHC) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN2854697) IHC-P Image GRB2 antibody detects GRB2 protein at nucleus on human colon carcinoma by...
Immunofluorescence (IF) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN2854697) ICC/IF Image GRB2 antibody detects GRB2 protein at nucleus by immunofluorescent analy...
Immunohistochemistry (IHC) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN2854697) IHC-P Image GRB2 antibody detects GRB2 protein at nucleus on human ovarian carcinoma ...
Immunohistochemistry (IHC) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN2854697) IHC-P Image GRB2 antibody detects GRB2 protein at nucleus on human breast carcinoma b...
Western Blotting (WB) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN2854697) WB Image Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE GRB2 antibody antib...
Immunohistochemistry (IHC) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN2854697) IHC-P Image GRB2 antibody detects GRB2 protein at nucleus on mouse lung by immunohist...