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|Antigen||Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies|
|Conjugate||This PDGFA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PDGFA AntibodyTarget Details PDGFA Application Details Handling Images
|Specificity||The antibody recognizes the human Platelet Derived Growth Factor AA (hPDGF-AA). Not tested in other species.|
|Purification||Immunoaffinity chromatography using immobilized human PDGF matrix|
|Immunogen||Highly pure (>98%) recombinant hPDGF-AA|
|Plasmids, Primers & others|
Target Details PDGFAProduct Details anti-PDGFA Antibody Application Details Handling Images back to top
|Alternative Name||PDGFA (PDGFA Antibody Abstract)|
|Background||Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling|
Application DetailsProduct Details anti-PDGFA Antibody Target Details PDGFA Handling Images back to top
Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/mL),a concentration of 0.05 - 0.07 μg/mL of this antibody is required. Immunohistochemistry: The antibody stained formalin-fixed, paraffin embedded sections of normal human skin at1.0 μg/mL overnight at 4C. (A HRP-labeled polymer detection system was used with anon-alcohol soluble AEC chromogen and a proteinase K antigen retrieval). ELISA: Indirect - To detect hPDGF-AA by indirect ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Sandwich - To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, in conjunction with abiotinylated anti-human PDGF-AA (PP1061B) as the detection antibody at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA can be detected. Western blot: To detect hPDGF-AA by Western blot analysis this antibody can be used at a concentrationof 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDGFA Antibody Target Details PDGFA Application Details Images back to top
|Reconstitution||Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2, without preservatives|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.|
|Expiry Date||12 months|