anti-Human PDGFA antibody for Enzyme Immunoassay

Recommended PDGFA Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
  • PDGF-A
  • PDGF1
  • pdgfa
  • pdgfa-2
  • zgc:100777
  • pdgf
  • pdgf1
  • pdgf-a
  • PDGFACP
  • wu:fc32c12
  • zgc:153249
  • platelet derived growth factor subunit A
  • platelet-derived growth factor alpha polypeptide a
  • platelet derived growth factor, alpha
  • platelet derived growth factor subunit A S homeolog
  • platelet-derived growth factor alpha polypeptide b
  • PDGFA
  • pdgfaa
  • pdgfa
  • Pdgfa
  • pdgfa.S
  • pdgfab
Reactivity
Human
151
60
57
5
1
1
1
1
1
1
Host
Rabbit
131
32
4
3
1
Clonality
Polyclonal
Conjugate
This PDGFA antibody is un-conjugated
21
15
6
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
135
75
54
38
29
15
12
11
8
5
3
3
2
2
2
2
1
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Catalog No. ABIN116207
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116206 EIA WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN116205 EIA WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN116208 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
Reactivity Human
(151), (60), (57), (5), (1), (1), (1), (1), (1), (1)
Host Rabbit
(131), (32), (4), (3), (1)
Clonality
Polyclonal
Conjugate This PDGFA antibody is un-conjugated
(21), (15), (6), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(135), (75), (54), (38), (29), (15), (12), (11), (8), (5), (3), (3), (2), (2), (2), (2), (1)
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Product Details anti-PDGFA Antibody

Target Details PDGFA Application Details Handling Images
Specificity The antibody recognizes the human Platelet Derived Growth Factor AA (hPDGF-AA). Not tested in other species.
Purification Immunoaffinity chromatography using immobilized human PDGF matrix
Immunogen Highly pure (>98%) recombinant hPDGF-AA
Plasmids, Primers & others

Target Details PDGFA

Product Details anti-PDGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDGFA (PDGFA Antibody Abstract)
Background Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A
Gene ID 5154
NCBI Accession NP_002598
UniProt P04085
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFA Antibody Target Details PDGFA Handling Images back to top
Application Notes Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/mL),a concentration of 0.05 - 0.07 μg/mL of this antibody is required. Immunohistochemistry: The antibody stained formalin-fixed, paraffin embedded sections of normal human skin at1.0 μg/mL overnight at 4C. (A HRP-labeled polymer detection system was used with anon-alcohol soluble AEC chromogen and a proteinase K antigen retrieval). ELISA: Indirect - To detect hPDGF-AA by indirect ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Sandwich - To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, in conjunction with abiotinylated anti-human PDGF-AA (PP1061B) as the detection antibody at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA can be detected. Western blot: To detect hPDGF-AA by Western blot analysis this antibody can be used at a concentrationof 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDGFA Antibody Target Details PDGFA Application Details Images back to top
Reconstitution Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2, without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
Expiry Date 12 months