|Antigen||Phospholipase C gamma 2 (PLCG2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Phospholipase C gamma 2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-Phospholipase C gamma 2 AntibodyTarget Details Phospholipase C gamma 2 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of PLCG2 protein. (region surrounding Arg747)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen|
Target Details Phospholipase C gamma 2Product Details anti-Phospholipase C gamma 2 Antibody Application Details Handling Images back to top
|Alternative Name||PLCG2 (PLCG2 Antibody Abstract)|
|Background||Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLCγ1, PLCγ2, PLC δ1, PLC δ2 and PLCe. PLCδ exists as four different isoforms. PLC δ1, a calcium signal amplifier, is activated by an atypical GTP-binding protein. In addition, PLC δ1 is an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and α1B-adrenoreceptor signaling. Mouse PLC δ1 is highly expressed in brain, heart, lung and testis. PLC δ is abnormally accumulated in autopsied brains with Alzheimer's disease (AD), suggesting that it may play a role in the pathology of AD. PLC δ2 is markedly expressed in type II intestinal metaplasia and in the adenocarcinoma. When PLC δ2 is expressed in type I intestinal metaplasia, the metaplasia is generally considered benignant, yet evolves toward neoplastic transformation. Thus, PLC δ2 expression may be a possible marker of gastric malignant transformation.Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2, PLC-IV, PLC-gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, Phospholipase C-gamma-2|
|Molecular Weight||approx. 148 kDa|
|Pathways||RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling|
Application DetailsProduct Details anti-Phospholipase C gamma 2 Antibody Target Details Phospholipase C gamma 2 Handling Images back to top
ELISA: 1/5000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Phospholipase C gamma 2 Antibody Target Details Phospholipase C gamma 2 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Phospholipase C gamma 2 Antibody Target Details Phospholipase C gamma 2 Application Details Handling back to top