anti-Chicken CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Chicken
1270
579
228
69
60
41
32
30
28
26
25
25
22
21
18
18
15
12
10
10
10
9
7
7
5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
1321
517
313
13
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to PE
248
215
152
127
41
40
38
32
30
29
28
25
24
20
16
15
15
15
15
15
15
14
14
13
10
10
9
8
8
8
8
7
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1644
540
471
301
285
270
215
165
91
73
49
39
31
26
21
19
18
14
14
11
11
9
7
6
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN135511
$ 330.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
22.905727 ABIN135509 FACS FITC Mouse IgG1 Log in to see CT-4 3
22.905727 ABIN135510 FACS Biotin Mouse IgG1 Log in to see CT-4 2
18.405727 ABIN135508 FACS Mouse IgG1 Log in to see CT-4 1
15.405727 ABIN135529 FACS FITC Mouse IgM Log in to see EP96 0
15.405727 ABIN135531 FACS PE Mouse IgM Log in to see EP96 0
15.405727 ABIN135530 FACS Biotin Mouse IgM Log in to see EP96 0
15.405727 ABIN135528 FACS Mouse IgM Log in to see EP96 0
15.405727 ABIN5707033 FACS APC Mouse IgG1 Log in to see CT-4 0
12.405727 ABIN288957 FACS IHC FITC Mouse IgG1 Log in to see CT-4 0
12.405727 ABIN288962 FACS IHC PE Mouse IgM Log in to see EP96 0
12.405727 ABIN288958 FACS IHC PE Mouse IgG1 Log in to see CT-4 0
12.405727 ABIN288961 FACS IHC FITC Mouse IgM Log in to see EP96 0
12.405727 ABIN288956 FACS IP IHC Biotin Mouse IgG1 Log in to see CT-4 0
12.405727 ABIN288960 FACS IP IHC Biotin Mouse IgM Log in to see EP96 0
12.405727 ABIN284270 FACS IP IHC Mouse IgG1 Log in to see CT-4 0
12.405727 ABIN284271 FACS IP IHC Mouse IgM Log in to see EP96 0
9.766454 ABIN371334 FACS PE Mouse IgG1 Log in to see CT-4 0
9.766454 ABIN371354 FACS FITC Mouse IgM Log in to see EP96 0
1.9425783 ABIN440225 BP FACS IHC IHC (fro) IHC (p) IP Neut Mouse IgG1 kappa Log in to see CT-4 0
1 ABIN593666 FACS PE Mouse IgG2b Log in to see 0

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Chicken
(1270), (579), (228), (69), (60), (41), (32), (30), (28), (26), (25), (25), (22), (21), (18), (18), (15), (12), (10), (10), (10), (9), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(1321), (517), (313), (13), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to PE
(248), (215), (152), (127), (41), (40), (38), (32), (30), (29), (28), (25), (24), (20), (16), (15), (15), (15), (15), (15), (15), (14), (14), (13), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1644), (540), (471), (301), (285), (270), (215), (165), (91), (73), (49), (39), (31), (26), (21), (19), (18), (14), (14), (11), (11), (9), (7), (6), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN135511) Images
Specificity Chicken/Turkey/Quail/Pigeon CD4, Mr 64 kDa
Characteristics Mouse Anti-Chicken CD4-PE
Immunogen Chicken thymocytes and Ig-negative blood leukocytes
Clone CT-4
Isotype IgG1
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN135511) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a type I transmembrane glycoprotein expressed on approximately 70 % of thymocytes, 10 % of spleen cells and 45 % of peripheral blood lymphocytes, but < 1 % of bursal cells.
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN135511) Images back to top
Application Notes
  • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature
  • Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF700, AF647, and APC conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume 1 mL
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN135511) Images back to top
Concentration 0.1 mg/mL
Buffer 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze!
Protect conjugated products from light.

Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store at 2-8°C

References for anti-CD4 antibody (ABIN135511)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Carballeda, Zoth, Gómez, Gravisaco, Berinstein: "Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain." in: Immunobiology, Vol. 216, Issue 9, pp. 1028-33, 2011

Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, 2010

Parvizi, Andrzejewski, Read, Behboudi, Sharif: "Expression profiling of genes associated with regulatory functions of T-cell subsets in Marek's disease virus-infected chickens." in: Avian pathology : journal of the W.V.P.A, Vol. 39, Issue 5, pp. 367-73, 2010

Background publications

Chan, Chen, Ager, Cooper: "Identification of the avian homologues of mammalian CD4 and CD8 antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 7, pp. 2133-8, 1988

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN135511) back to top
Supplier Images
 image for anti-CD4 Molecule (CD4) antibody (PE) (ABIN135511) anti-CD4 Molecule (CD4) antibody (PE)
 image for anti-CD4 Molecule (CD4) antibody (PE) (ABIN135511) Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD4-PE.