|Antigen||CD4 Molecule (CD4) Antibodies|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
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Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling Images
|Specificity||Reacts with CD4 antigen. Has been assigned to the CD4 cluster of differentiation at the 3rd International Workshop in Boston (1986). The CD4 antigen is present on approximately 45% of peripheral blood lymphocytes (T4 lymphocytes), 80% of thymocytes and with a lower density on 100% of monocytes and on some neutrophils.|
|Characteristics||MAb to CD4, Monoclonal antibody to Human CD4|
|Purification||Protein A Chromatography|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling Images back to top
|Application Notes||Suitable for use in flow cytometry (1:15-1:30). Use 20-l working dilution per/5x105 cells/test or 100 µL blood sample. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.|
|Restrictions||For Research Use only|
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|Reconstitution||Reconstitute with 1 mL distilled water.|
|Buffer||Lyophilized from PBS containing 1 mg/mL BSA.|
Avoid multiple freeze/thaw cycles. The addition of 0.1 % (w/v) sodium azide is recommended for storage of the Reconstituted form for up to one month at 2-8 °C.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
|Storage||4 °C/-20 °C|
|Storage Comment||Store lyophilized product at 2-8 °C. After reconstitution aliquot and store at -28 °C.|