anti-Human CD4 antibody for Immunoprecipitation

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Epitope
Domain D1
69
53
29
21
16
15
15
15
13
9
8
8
6
6
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1272
584
237
71
61
41
36
30
28
26
25
25
22
21
18
18
15
15
13
12
10
9
9
7
5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
1348
525
303
13
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
253
223
158
133
44
41
39
32
32
32
29
25
24
21
16
16
16
15
15
15
15
15
15
13
10
10
9
8
8
8
8
7
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
1658
546
462
299
287
274
217
166
94
72
54
39
31
26
21
19
18
14
14
11
11
9
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN125679
$ 349.14
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
29.462074 ABIN94096 Func FACS IP Mouse IgG2a Domain D1 Log in to see MEM-115 6
21.755764 ABIN5668250 FACS IF IHC (p) IP WB Rabbit IgG, kappa Log in to see Chimeric 0
17.255764 ABIN94103 FACS IP Mouse IgM Domain D1 Log in to see MEM-16 4
15.755764 ABIN5710597 FACS Func IP WB Mouse IgG2a Log in to see EM4 0
15.755764 ABIN2144257 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
15.755764 ABIN2144344 FACS IF IP IHC PE-Cy5 Mouse IgG2b Log in to see 4AOKT4 2
15.755764 ABIN2144256 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
15.755764 ABIN2144254 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
15.755764 ABIN2144255 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
15.755764 ABIN2144346 FACS IF IP IHC PE-Cy5 Mouse IgG2b Log in to see 4AOKT4 2
15.755764 ABIN2144750 FACS IF IP IHC PE Mouse IgG2b Log in to see 4AOKT4 2
15.755764 ABIN2144459 FACS IF IP IHC FITC Mouse IgG2b Log in to see 4AOKT4 2
15.755764 ABIN2144752 FACS IF IP IHC PE Mouse IgG2b Log in to see 4AOKT4 2
15.755764 ABIN2144461 FACS IF IP IHC FITC Mouse IgG2b Log in to see 4AOKT4 2
12.755764 ABIN5668249 FACS IF IHC (p) IP WB Mouse IgG1, kappa Log in to see MT310 0
12.755764 ABIN2144207 FACS IF IP IHC APC Mouse IgG2b Log in to see 4AOKT4 2
12.755764 ABIN2144209 FACS IF IP IHC APC Mouse IgG2b Log in to see 4AOKT4 2
12.755764 ABIN288968 FACS IP IHC Biotin Mouse IgG1 Log in to see RFT-4G 0
12.755764 ABIN284274 FACS IP IHC Mouse IgG1 Log in to see RFT-4g 0
12.755764 ABIN2967172 Func FACS IP Mouse IgG2a Log in to see MEM-115 0

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Domain D1
(69), (53), (29), (21), (16), (15), (15), (15), (13), (9), (8), (8), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1272), (584), (237), (71), (61), (41), (36), (30), (28), (26), (25), (25), (22), (21), (18), (18), (15), (15), (13), (12), (10), (9), (9), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(1348), (525), (303), (13), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(253), (223), (158), (133), (44), (41), (39), (32), (32), (32), (29), (25), (24), (21), (16), (16), (16), (15), (15), (15), (15), (15), (15), (13), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(1658), (546), (462), (299), (287), (274), (217), (166), (94), (72), (54), (39), (31), (26), (21), (19), (18), (14), (14), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN125679) Images
Specificity The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“,helper“, T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.
HLDA V, WS Code T T-CD04.09
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Immunogen Human thymocytes and T lymphocytes.
Clone MEM-115
Isotype IgG2a
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN125679) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN125679) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN125679) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD4 antibody (ABIN125679)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Bosze, Caccamo, Majer, Mezo, Dieli, Hudecz: "In vitro T-cell immunogenicity of oligopeptides derived from the region 92-110 of the 16-kDa protein of Mycobacterium tuberculosis." in: Biopolymers, Vol. 76, Issue 6, pp. 467-76, 2004

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003

Singer, Scott, Kawka, Chin, Daugherty, DeMartino, DiSalvo, Gould, Lineberger, Malkowitz, Miller, Mitnaul, Siciliano, Staruch, Williams, Zweerink, Springer: "CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of human macrophages and T cells." in: Journal of virology, Vol. 75, Issue 8, pp. 3779-90, 2001

Background publications

Clapham, McKnight: "Cell surface receptors, virus entry and tropism of primate lentiviruses." in: The Journal of general virology, Vol. 83, Issue Pt 8, pp. 1809-29, 2002

Foti, Phelouzat, Holm, Rasmusson, Carpentier: "p56Lck anchors CD4 to distinct microdomains on microvilli." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 4, pp. 2008-13, 2002

Millán, Cerny, Horejsi, Alonso: "CD4 segregates into specific detergent-resistant T-cell membrane microdomains." in: Tissue antigens, Vol. 53, Issue 1, pp. 33-40, 1999

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN125679) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-CD4 Molecule (CD4) (Domain D1) antibody (ABIN125679) Immunoprecipitation of human CD4 from the lysate T cells isolated from fresh buffy co...