anti-Human CD4 antibody for Western Blotting

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Epitope
AA 420-457
69
53
30
20
16
15
15
14
13
9
8
8
6
6
6
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1261
576
227
69
61
41
34
30
28
26
25
25
22
21
18
18
15
13
11
10
10
9
8
7
5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
1324
517
305
13
4
2
1
1
Clonality
Polyclonal
Conjugate
This CD4 antibody is un-conjugated
247
216
153
127
41
40
38
32
30
29
29
25
24
20
16
15
15
15
15
15
15
14
14
13
10
10
9
8
8
8
8
7
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1649
531
460
302
281
268
214
166
91
73
49
38
31
26
21
19
18
14
14
11
11
9
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Options
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Available images

Catalog No. ABIN671376
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.25679 ABIN94089 FACS MCA WB Biotin Mouse IgG1 N-Term Log in to see MEM-241 9
25.25679 ABIN94088 FACS MCA WB Mouse IgG1 N-Term Log in to see MEM-241 10
25.25679 ABIN192276 FACS MCA WB PE-DyLight 594 Mouse IgG1 N-Term Log in to see MEM-241 9
25.25679 ABIN302054 FACS MCA WB PerCP Mouse IgG1 N-Term Log in to see MEM-241 9
25.25679 ABIN94090 FACS MCA WB FITC Mouse IgG1 N-Term Log in to see MEM-241 9
25.25679 ABIN94092 FACS MCA WB APC Mouse IgG1 N-Term Log in to see MEM-241 9
25.25679 ABIN94091 FACS MCA WB PE Mouse IgG1 N-Term Log in to see MEM-241 9
22.25679 ABIN5668250 FACS IF IHC (p) IP WB Rabbit IgG, kappa Log in to see Chimeric 0
21.281914 ABIN439030 ICC IF IHC IHC (p) SimWes WB Rabbit C-Term, AA 400-458 Log in to see Polyclonal 14
20.063175 ABIN3183771 ELISA IF IHC WB Rabbit IgG Ser213 Log in to see Polyclonal 0
17.75679 ABIN965794 IHC ELISA WB IgG1 Log in to see 1
17.063175 ABIN2854871 WB Rabbit IgG Center Log in to see Polyclonal 0
17.063175 ABIN3022125 IHC WB Rabbit IgG Log in to see Polyclonal 0
17.063175 ABIN929130 WB Rabbit C-Term Log in to see Polyclonal 0
17.063175 ABIN6254232 WB Goat IgG Log in to see Polyclonal 0
17.063175 ABIN5663636 WB Rabbit Log in to see Polyclonal 0
16.806385 ABIN5996946 IF/ICC IHC WB Rabbit IgG Log in to see 0
16.25679 ABIN5668246 ELISA EIA FACS IHC (fro) WB Rabbit IgG, kappa Log in to see Chimeric 0
16.25679 ABIN5710597 FACS Func IP WB Mouse IgG2a Log in to see EM4 0
16.25679 ABIN3209995 FACS WB Mouse IgG1 Log in to see B486A1 0

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope AA 420-457
(69), (53), (30), (20), (16), (15), (15), (14), (13), (9), (8), (8), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1261), (576), (227), (69), (61), (41), (34), (30), (28), (26), (25), (25), (22), (21), (18), (18), (15), (13), (11), (10), (10), (9), (8), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(1324), (517), (305), (13), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This CD4 antibody is un-conjugated
(247), (216), (153), (127), (41), (40), (38), (32), (30), (29), (29), (25), (24), (20), (16), (15), (15), (15), (15), (15), (15), (14), (14), (13), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1649), (531), (460), (302), (281), (268), (214), (166), (91), (73), (49), (38), (31), (26), (21), (19), (18), (14), (14), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN671376) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from rat CD4
Isotype IgG
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN671376) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Subcellular location: Cytoplasm

Synonyms: p55, W3/25, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen, CD4

Gene ID 24932
UniProt P05540
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN671376) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN671376) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-CD4 antibody (ABIN671376)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Smit, Bester, van den Heever, Schlegel, Botes, Dohmen: "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?" in: Cell and tissue banking, 2015 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human). Further details: Sample: Heart

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN671376) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclona...
Immunofluorescence (IF) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibo...
Immunohistochemistry (IHC) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgica...
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Mouse spleen cells probed with Rabbit Anti-CD4 Polyclonal Antibody, PE Conjugated fo...
Western Blotting (WB) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with at 1:5000 90min...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in...
'Independent Validation' Badge
Lot Number 130703
Method validated Immunohistochemistry
Positive Control Mouse spleen tissue
Negative Control Mouse muscle tissue
Notes Signal was detected in positive control sample and not in negative control sample.
Primary Antibody
  • Antibody: CD4 Molecule (CD4)
  • Catalog number: ABIN671376
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 130703
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: Spleen tissue (specimen known to contain the target protein) from mouse.
  • Negative Control: Muslce tissue (specimen known to not contain the target protein) from mouse.
  • Primary antibody isotype control: Mouse spleen treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Mouse spleen treated with secondary antibody only (no primary antibody).
Protocol
  • Immunohistochemistry was performed manually.
  • Sections were deparaffinized and rehydrated.
  • Sections were heated to 98°C for 10 min in 0.1M Borate buffer with 1 mM NaCl and 1 mM EDTA pH 8.0 for antigen retrieval.
  • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidases.
  • Sections were washed x 3 in Tris buffered saline (TBS).
  • Sections were blocked in 5 % normal donkey serum for 60 min at room temperature.
  • Sections were incubated with primary antibody diluted 1:200 in 5% normal donkey serum in TBS. Incubated at 4°C temperature overnight.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature..
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
  • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 sec at RT.
  • Sections were washed x 3 in Distilled Water.
  • Sections were counterstained with hematoxylin for 20 sec.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
Experimental Notes - No experimental challenges noted.
Validation Images
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 1: Mouse spleen stained with anti-CD4 (brown) and counterstained with hematoxy...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 2: Mouse muscle stained with anti-CD4 (brown) and counterstained with hematoxy...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 3: Mouse spleen stained with isotype control antibody (brown) and counterstain...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 4: Mouse spleen stained with secondary antibody only (brown) and counterstaine...