anti-Rat (Rattus) CD4 antibody for Immunoprecipitation

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Rat (Rattus)
1230
639
247
79
68
42
36
29
26
26
22
21
19
18
16
16
15
12
10
9
8
7
6
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
Host
Mouse
1386
580
286
10
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
267
227
168
140
49
49
48
41
34
34
30
29
26
23
22
22
20
19
19
19
19
16
15
15
13
13
13
11
10
9
8
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
1758
474
456
356
320
297
170
170
98
79
39
38
31
31
30
25
18
14
14
11
11
9
6
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN2689228
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2689227 IHC (fro) FACS IP Mouse IgG2a, kappa Log in to see OX 8
1 ABIN120383 FACS IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 8
1 ABIN2480228 IHC (fro) FACS IP Mouse IgG2a Domain 1 Log in to see OX-38 4
1 ABIN2661701 FACS IP IHC WB FITC Mouse IgG1 kappa Log in to see W3-25 6
1 ABIN2664660 BR IP IHC ELISA WB Mouse IgG1 kappa Log in to see W3-25 6
1 ABIN120380 FACS IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 2
1 ABIN206563 FACS IHC (fro) IF IP ELISA Mouse IgG2a Log in to see RIV6 0
1 ABIN2480233 FACS IF IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 4
1 ABIN206159 FACS IHC (fro) IP Mouse IgG2a Log in to see 0
1 ABIN120381 FACS IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 1
1 ABIN213133 FACS IHC (fro) IP Mouse IgG2a Log in to see OX-38 0
1 ABIN2225958 FACS IP IHC Mouse IgG2a Log in to see 3H733 0
1 ABIN210173 FACS IHC (fro) IP Mouse IgG2a Log in to see 0
1 ABIN1854063 FACS IF/ICC IP FITC Mouse IgG2a Log in to see 0
1 ABIN1854065 FACS IF/ICC IP PE Mouse IgG2a Log in to see 0
1 ABIN1854064 FACS IF/ICC IP Biotin Mouse IgG2a Log in to see 0
1 ABIN2900378 FACS IF/ICC IP APC Mouse IgG2a Log in to see 0
1 ABIN2900379 FACS IF/ICC IP PerCP Mouse IgG2a Log in to see 0
1 ABIN5558707 ELISA IHC IP WB Biotin Rabbit C-Term Log in to see Polyclonal 0

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Rat (Rattus)
(1230), (639), (247), (79), (68), (42), (36), (29), (26), (26), (22), (21), (19), (18), (16), (16), (15), (12), (10), (9), (8), (7), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1386), (580), (286), (10), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(267), (227), (168), (140), (49), (49), (48), (41), (34), (34), (30), (29), (26), (23), (22), (22), (20), (19), (19), (19), (19), (16), (15), (15), (13), (13), (13), (11), (10), (9), (8), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(1758), (474), (456), (356), (320), (297), (170), (170), (98), (79), (39), (38), (31), (31), (30), (25), (18), (14), (14), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
Supplier Log in to see

Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN2689228) Images
Brand BD Pharmingen™
Characteristics The OX-38 antibody has been reported to react with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, and some dendritic cells. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. The OX-38 antibody has been reported to bind to the same epitope of CD4 as that recognized by W3/25 mAb, which is a different epitope than that recognized by OX-35 mAb (Cat. No. 554837). In vivo blocking of some cell-mediated immune responses by mAb OX-38 has been reported. Injection of OX-38 mAb induces allograft unresponsiveness in rats, with varying results depending on the rat strain used (high or low responder). Furthermore, in vivo depletion of CD4+ lymphocytes has been reported with this antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD4 on rat splenocytes. Lewis splenocytes were incubated simultaneously with PE-conjugated anti-rat CD3 G4.18 (Cat. No. 554833) and purified OX-38 (right panel), followed by FITC anti-mouse IgG2a R19-15 (Cat. No. 553390) monoclonal antibodies. The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD4 - Purified - Clone OX-38 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat thymocyte glycoproteins
Clone OX
Isotype IgG2a, kappa

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN2689228) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN2689228) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN2689228) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD4 antibody (ABIN2689228)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Liu, Zhang, Jenkins, MacPherson: "Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, 1998

Suzuki, Hara, Miyahara, Tanigawa, Matsuzaki, Onitsuka, Shibata, Koga, Yamashita, Miyamoto, Suzuki, Amemiya, Iwaya, Miyasaka, Kimura: "Microchimerism and graft acceptance: IV. Cardiac allograft acceptance following anti-adhesion molecule antibody therapy." in: Transplantation proceedings, Vol. 28, Issue 4, pp. 2058-60, 1996

Yin, Fathman: "Tissue-specific effects of anti-CD4 therapy in induction of allograft unresponsiveness in high and low responder rats." in: Transplant immunology, Vol. 3, Issue 3, pp. 258-64, 1996

Arima, Goss, Walp, Flye: "Administration of anti-CD4 monoclonal antibody with intrathymic injection of alloantigen results in rat cardiac allograft tolerance." in: Surgery, Vol. 118, Issue 2, pp. 265-72; discussion 272-3, 1995

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." in: Immunology, Vol. 79, Issue 2, pp. 298-304, 1993

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Stitz, Sobbe, Bilzer: "Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats." in: Journal of virology, Vol. 66, Issue 6, pp. 3316-23, 1992

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, 1985