anti-Rat (Rattus) CD4 antibody for Immunoprecipitation

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Epitope
Domain 2
66
65
21
19
15
15
15
13
12
10
9
8
8
8
8
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
1225
631
233
68
67
44
36
27
26
22
21
19
18
16
15
15
13
10
10
9
8
7
6
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
Host
Mouse
1380
588
285
11
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
265
227
168
139
50
49
47
41
34
34
30
28
26
22
21
21
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
1758
496
448
360
326
309
162
158
88
78
39
38
32
31
30
25
18
14
14
11
11
9
6
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN120383
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120380 FACS IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 2
1 ABIN2661701 FACS IP IHC WB FITC Mouse IgG1 kappa Log in to see W3-25 6
1 ABIN120381 FACS IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 1
1 ABIN2480228 IHC (fro) FACS IP Mouse IgG2a Domain 1 Log in to see OX-38 4
1 ABIN2664660 BR IP IHC ELISA WB Mouse IgG1 kappa Log in to see W3-25 6
1 ABIN206563 FACS IHC (fro) IF IP ELISA Mouse IgG2a Log in to see RIV6
1 ABIN2480233 FACS IF IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 4
1 ABIN2689227 IHC (fro) FACS IP Mouse IgG2a, kappa Log in to see OX 8
1 ABIN2689228 BR IHC (fro) FACS IP Mouse IgG2a, kappa Log in to see OX 9
1 ABIN213133 FACS IHC (fro) IP Mouse IgG2a Log in to see OX-38
1 ABIN206159 FACS IHC (fro) IP Mouse IgG2a Log in to see
1 ABIN210173 FACS IHC (fro) IP Mouse IgG2a Log in to see
1 ABIN2225958 FACS IP IHC Mouse IgG2a Log in to see 3H733
1 ABIN1854063 FACS IF/ICC IP FITC Mouse IgG2a Log in to see
1 ABIN1854064 FACS IF/ICC IP Biotin Mouse IgG2a Log in to see
1 ABIN1854065 FACS IF/ICC IP PE Mouse IgG2a Log in to see
1 ABIN2900378 FACS IF/ICC IP APC Mouse IgG2a Log in to see
1 ABIN2900379 FACS IF/ICC IP PerCP Mouse IgG2a Log in to see
1 ABIN5558707 ELISA IHC IP WB Biotin Rabbit C-Term Log in to see Polyclonal

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Domain 2
(66), (65), (21), (19), (15), (15), (15), (13), (12), (10), (9), (8), (8), (8), (8), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(1225), (631), (233), (68), (67), (44), (36), (27), (26), (22), (21), (19), (18), (16), (15), (15), (13), (10), (10), (9), (8), (7), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1380), (588), (285), (11), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(265), (227), (168), (139), (50), (49), (47), (41), (34), (34), (30), (28), (26), (22), (21), (21), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(1758), (496), (448), (360), (326), (309), (162), (158), (88), (78), (39), (38), (32), (31), (30), (25), (18), (14), (14), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN120383) Images
Purification Affinity chromatography on Protein G
Immunogen MLR generated rat T helper lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone OX-35
Isotype IgG2a

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN120383) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 10116
UniProt P05540
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN120383) Images back to top
Application Notes Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN120383) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD4 antibody (ABIN120383)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Wang, Wu, Shieh, Wen, Ling: "Immunohistochemical study of amoeboid microglial cells in fetal rat brain." in: Journal of anatomy, Vol. 189 ( Pt 3), pp. 567-74, 1997

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Charteris, Lightman: "In vivo lymphokine production in experimental autoimmune uveoretinitis." in: Immunology, Vol. 78, Issue 3, pp. 387-92, 1993

Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, 1988

Hayosh, Swanborg: "Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 11, pp. 3771-5, 1987

Tellides, Dallman, Kupiec-Weglinski, Diamantstein, Morris: "Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy." in: Transplantation proceedings, Vol. 19, Issue 5, pp. 4231-3, 1987

Signore, Parman, Pozzilli, Andreani, Beverley: "Detection of activated lymphocytes in endocrine pancreas of BB/W rats by injection of 123I-interleukin-2: an early sign of type 1 diabetes." in: Lancet, Vol. 2, Issue 8558, pp. 537-40, 1987

Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, 1985