anti-Human LAT antibody for Immunocytochemistry

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
  • linker for activation of T-cells
  • linker for activation of T cells
  • LAT
  • lat
  • Lat
Epitope
AA 242-262, C-Term
31
24
22
22
14
7
5
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
277
122
103
3
2
1
Host
Rabbit
167
103
13
2
Clonality
Polyclonal
Conjugate
This LAT antibody is un-conjugated
9
9
8
7
7
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
213
81
51
49
39
33
32
9
6
3
2
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN3043991
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1868912 ICC IHC WB Rabbit IgG AA 25-221 Log in to see Polyclonal 0
1 ABIN6272038 ELISA ICC IF WB Rabbit IgG pTyr255 Log in to see Polyclonal 0
1 ABIN6271566 ELISA ICC IF IHC WB Rabbit IgG pTyr161 Log in to see Polyclonal 0
1 ABIN2624774 ICC IHC IHC (p) WB Rabbit IgG AA 242-262 Log in to see Polyclonal 0
1 ABIN6262916 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN3031630 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Epitope AA 242-262, C-Term
(31), (24), (22), (22), (14), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(277), (122), (103), (3), (2), (1)
Host Rabbit
(167), (103), (13), (2)
Clonality
Polyclonal
Conjugate This LAT antibody is un-conjugated
(9), (9), (8), (7), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(213), (81), (51), (49), (39), (33), (32), (9), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-LAT Antibody

Target Details LAT Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Linker for activation of T-cells family member 1(LAT) detection. Tested with WB, IHC-P, ICC in Human.
Sequence QEAEEVEEEG APDYENLQEL N
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Linker for activation of T-cells family member 1(LAT) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: linker for activation of T cells
Protein Name: Linker for activation of T-cells family member 1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human LAT(242-262aa QEAEEVEEEGAPDYENLQELN), different from the related mouse and rat sequences by six amino acids.
Isotype IgG
Plasmids, Primers & others

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background Linker for activation of T cells, also known as LAT, is a protein which in humans is encoded by the LAT gene. This gene is mapped to 16p11.2. The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor(TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement.

Synonyms: 36 kDa phospho tyrosine adapter protein antibody|36 kDa phospho-tyrosine adapter protein antibody|36 kDa phospho-tyrosine adaptor protein antibody|LAT 1 antibody|lat antibody|LAT_HUMAN antibody|LAT1 antibody|Linker for activation of T cells antibody|Linker for activation of T cells family member 1 antibody|linker for activation of T cells, transmembrane adaptor antibody|Linker for activation of T-cells family member 1 antibody|p36 38 antibody|p36-38 antibody|pp36 antibody
UniProt O43561
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for LAT is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Linker For Activation of T Cells (LAT) (AA 242-262), (C-Term) antibody (ABIN3043991) Anti-LAT antibody, IHC(P) IHC(P): Human Appendicitis Tissue
Western Blotting (WB) image for anti-Linker For Activation of T Cells (LAT) (AA 242-262), (C-Term) antibody (ABIN3043991) Anti-LAT antibody, Western blotting All lanes: Anti LAT at 0.5ug/ml Lane 1: Human Pl...
Western Blotting (WB) image for anti-Linker For Activation of T Cells (LAT) (AA 242-262), (C-Term) antibody (ABIN3043991) Anti-LAT antibody, Western blottingAll lanes: Anti LAT at 0.5ug/ml Lane 1: Human Pla...