anti-Human LAT antibody for Immunohistochemistry

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
  • linker for activation of T-cells
  • linker for activation of T cells
  • LAT
  • lat
  • Lat
Epitope
AA 1-234
31
24
22
22
13
10
7
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
280
129
112
3
2
1
Host
Mouse
163
124
12
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
11
11
8
8
7
6
6
6
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
218
72
60
50
39
35
28
20
13
6
2
2
1
1
1
1
1
1
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Catalog No. ABIN393665
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.593313 ABIN1531336 IHC ELISA WB Rabbit IgG AA 191-240, pTyr191 Log in to see Polyclonal 0
8.593313 ABIN1102812 IHC IHC (p) WB Rabbit AA 70-120 Log in to see Polyclonal 0
8.593313 ABIN2470864 IP IHC WB Mouse IgG1 kappa Log in to see LAT-10-17 (10-17) 0
8.593313 ABIN2470080 IP IHC WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT1111 0
1 ABIN293627 IHC IHC (p) IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT1111 0
1 ABIN573989 IHC IHC (p) IP WB Mouse IgG1, kappa Log in to see LAT-10-17 (10-17) 0
1 ABIN599213 ELISA IHC IHC (p) WB Mouse IgG1, kappa AA 1-234 Log in to see 3B11-1D5 0
1 ABIN1876851 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1868912 ICC IHC WB Rabbit IgG AA 25-221 Log in to see Polyclonal 0
1 ABIN3182545 ELISA IHC WB Rabbit IgG pTyr191 Log in to see Polyclonal 0
1 ABIN2624774 ICC IHC IHC (p) WB Rabbit IgG AA 242-262 Log in to see Polyclonal 0
1 ABIN342762 IHC ELISA WB Rabbit AA 29-49, N-Term Log in to see Polyclonal 0
1 ABIN2883990 ELISA IHC IHC (p) WB Rabbit IgG pTyr191 Log in to see Polyclonal 0
1 ABIN1819430 IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN2881203 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2886212 IHC WB Rabbit pTyr191 Log in to see Polyclonal 0
1 ABIN2297626 IC IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN4330362 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5618163 ELISA IHC WB Rabbit IgG pTyr191 Log in to see Polyclonal 0
1 ABIN2698119 IHC WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Epitope AA 1-234
(31), (24), (22), (22), (13), (10), (7), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(280), (129), (112), (3), (2), (1)
Host Mouse
(163), (124), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(11), (11), (8), (8), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(218), (72), (60), (50), (39), (35), (28), (20), (13), (6), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN393665) Images
Specificity LAT (AAH11563, 1 a.a. ~ 234 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: LAT (AAH11563, 1 a.a. ~ 233 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 3B11-1D5
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT antibody (ABIN393665) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background

Other Names: Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein, pp36, p36-38, LAT

Gene Description: linker for activation of T cells

Gene Summary: The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq

Molecular Weight NONE?! DA
Gene ID 27040
NCBI Accession NP_001014987, NP_001014988, NP_001014989, NP_055202
UniProt O43561
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT antibody (ABIN393665) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT antibody (ABIN393665) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-LAT antibody (ABIN393665)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Background publications

Davila, Froeling, Tan, Bonnard, Boland, Snippe, Hibberd, Seielstad: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010

Sowa, Bennett, Gygi, Harper: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009

Cruz-Orcutt, Houtman: "PI3 kinase function is vital for the function but not formation of LAT-mediated signaling complexes." in: Molecular immunology, Vol. 46, Issue 11-12, pp. 2274-83, 2009

Shen, Zhu, Lau, Chuck, Zhang: "The essential role of LAT in thymocyte development during transition from the double-positive to single-positive stage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 9, pp. 5596-604, 2009

Saito, Kawamoto, Kamatani: "Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects." in: Journal of human genetics, Vol. 54, Issue 6, pp. 317-23, 2009

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN393665) back to top
Supplier Images
Western Blotting (WB) image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) LAT monoclonal antibody (M01), clone 3B11-1D5 Western Blot analysis of LAT expression...
Immunohistochemistry (IHC) image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (Image 2)
Western Blotting (WB) image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Immunohistochemistry (IHC) image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) Immunoperoxidase of monoclonal antibody to LAT on formalin-fixed paraffin-embedded hu...
ELISA image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) Detection limit for recombinant GST tagged LAT is approximately 0.03ng/ml as a captur...