anti-Human LAT antibody for Immunoprecipitation

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
  • linker for activation of T-cells
  • linker for activation of T cells
  • LAT
  • lat
  • Lat
Epitope
Cytoplasmic Domain
30
24
22
20
15
7
6
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
263
124
104
3
2
1
Host
Mouse
173
82
13
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
8
8
7
6
6
5
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
201
80
39
35
35
33
30
9
6
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN302069
$ 394.53
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN573989 IHC IHC (p) IP WB Mouse IgG1, kappa Log in to see LAT-10-17 (10-17) 0
10 ABIN614546 IHC (p) IP WB Mouse IgG1 Log in to see LAT-10-17 (10-17) 0
8.5 ABIN535482 FACS IP WB Mouse IgG1 Log in to see LAT-01 3
7 ABIN293627 IHC IHC (p) IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT1111 0
7 ABIN5582401 IHC (p) IP WB Mouse IgG1 Log in to see LAT1111 0
7 ABIN5582400 IHC (p) IP WB Mouse IgG1 kappa Log in to see LAT-10-17 (10-17) 0
7 ABIN1724096 IHC (p) IP WB Mouse IgG1 kappa Log in to see LAT-10-17 (10-17) 0
4 ABIN2470864 IP IHC WB Mouse IgG1 kappa Log in to see LAT-10-17 (10-17) 0
4 ABIN2470080 IP IHC WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT1111 0
4 ABIN1724041 IHC (p) IP WB Mouse IgG1 Log in to see LAT1111 0
1 ABIN207036 FACS IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01 0
1 ABIN1821202 IP WB Mouse IgG1, kappa AA 31-233 Log in to see 0
1 ABIN120717 FACS IP WB Mouse IgG1 Log in to see LAT-01 0
1 ABIN233299 IP WB Rabbit IgG pTyr191 Log in to see Polyclonal 0
1 ABIN233300 IP WB Rabbit IgG pTyr171 Log in to see Polyclonal 0
1 ABIN1837825 IP WB Mouse IgG1 Log in to see LAT-01 0
1 ABIN2297617 IP WB Mouse IgG AA 31-233 Log in to see 1-T-2 0
1 ABIN3200681 FACS IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01 0
1 ABIN6720757 IP WB Rabbit IgG AA 31-233 Log in to see Polyclonal 0
1 ABIN2769567 IP IHC (p) WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT1111 0

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Epitope Cytoplasmic Domain
(30), (24), (22), (20), (15), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(263), (124), (104), (3), (2), (1)
Host Mouse
(173), (82), (13), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(8), (8), (7), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(201), (80), (39), (35), (35), (33), (30), (9), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN302069) Images
Specificity The antibody LAT-01 reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.
Clone LAT-01
Isotype IgG1
Plasmids, Primers & others

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT antibody (ABIN302069) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT antibody (ABIN302069) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT antibody (ABIN302069) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-LAT antibody (ABIN302069)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Product cited in:

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, 2005

Background publications

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Simeoni, Smida, Posevitz, Schraven, Lindquist: "Right time, right place: the organization of membrane proximal signaling." in: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN302069) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Linker For Activation of T Cells (LAT) (Cytoplasmic Domain) antibody (ABIN302069) Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-...