anti-Mouse (Murine) LAT antibody for Immunoprecipitation

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
  • linker for activation of T-cells
  • linker for activation of T cells
  • LAT
  • lat
  • Lat
Reactivity
Mouse (Murine), Rat (Rattus)
281
128
111
3
2
1
Host
Mouse
163
124
12
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
11
11
8
8
7
6
6
6
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
218
73
60
50
39
34
29
19
13
6
2
2
1
1
1
1
1
1
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Catalog No. ABIN94420
$ 290.95
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4330394 IP WB Mouse IgG1 AA 31-233 Log in to see 11B-12 0
1 ABIN1821202 IP WB Mouse IgG1, kappa AA 31-233 Log in to see 0
1 ABIN4330360 ICC IF IP WB Mouse IgG2a Log in to see LAT-1D1 0
1 ABIN3200682 ICC IP WB Mouse IgG2a full length Log in to see LAT-1D1 0
1 ABIN4330374 ICC IF IP WB PerCP Mouse IgG2a Log in to see LAT-1D1 0
1 ABIN4330376 ICC IF IP WB APC Mouse IgG2a Log in to see LAT-1D1 0
1 ABIN3038905 IF/ICC IP WB Mouse IgG2a Log in to see 0
1 ABIN4330372 ICC IF IP WB PE Mouse IgG2a Log in to see LAT-1D1 0

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(281), (128), (111), (3), (2), (1)
Host Mouse
(163), (124), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(11), (11), (8), (8), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(218), (73), (60), (50), (39), (34), (29), (19), (13), (6), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN94420) Images
Specificity The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
No Cross-Reactivity Human
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially expressed full-length rat LAT
Clone LAT-1D1
Isotype IgG2a
Plasmids, Primers & others

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT antibody (ABIN94420) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT antibody (ABIN94420) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT antibody (ABIN94420) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-LAT antibody (ABIN94420)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Background publications

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Product cited in:

Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, 2007

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, 2006

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004

Tolar, T?mová, Dráber: "New monoclonal antibodies recognizing the adaptor protein LAT." in: Folia biologica, Vol. 47, Issue 6, pp. 215-7, 2001

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN94420) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-Linker For Activation of T Cells (LAT) antibody (ABIN94420) Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets...
Immunocytochemistry (ICC) image for anti-Linker For Activation of T Cells (LAT) antibody (ABIN94420) Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets...