anti-Cow (Bovine) PAG1 antibody for Western Blotting

Recommended PAG1 Antibody (supplied by: Log in to see )

Antigen
phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
  • Cbp
  • F730007C19Rik
  • Pag
  • CBP
  • PAG
  • MGC80192
  • phosphoprotein associated with glycosphingolipid microdomains 1
  • phosphoprotein membrane anchor with glycosphingolipid microdomains 1
  • phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog
  • Pag1
  • PAG1
  • pag1.L
  • pag1
Epitope
C-Term
32
24
16
4
2
2
2
2
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
100
62
46
25
2
2
1
1
1
Host
Mouse
56
50
Clonality
Monoclonal
Conjugate
This PAG1 antibody is un-conjugated
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
69
55
47
33
24
13
7
6
3
1
1
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Catalog No. ABIN94270
$ 428.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN351516 IHC IHC (p) IP WB Mouse IgG2b C-Term Log in to see PAG-C1 0
4.727556 ABIN2789626 WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN3200740 IHC (p) IP WB Mouse IgG2b C-Term Log in to see PAG-C1 0
-2.0396347 ABIN269119 IHC (p) IP IHC WB Mouse IgG2b C-Term Log in to see PAG-C1 1
-12.539635 ABIN4343414 IHC IHC (p) WB Alexa Fluor 700 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343420 IHC IHC (p) WB DyLight 488 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343419 IHC IHC (p) WB DyLight 680 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343416 IHC IHC (p) WB Biotin Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343411 IHC IHC (p) WB Alexa Fluor 405 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343422 IHC IHC (p) WB DyLight 755 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343425 IHC IHC (p) WB DyLight 550 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343426 IHC IHC (p) WB DyLight 350 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343427 IHC IHC (p) WB DyLight 405 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343412 IHC IHC (p) WB Alexa Fluor 488 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343413 IHC IHC (p) WB Alexa Fluor 647 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343417 IHC IHC (p) WB DyLight 650 Mouse IgG2b C-Term Log in to see PAG-C1 0
-12.539635 ABIN4343421 IHC IHC (p) WB HRP Mouse IgG2b C-Term Log in to see PAG-C1 0

General

Antigen phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
Epitope C-Term
(32), (24), (16), (4), (2), (2), (2), (2), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
(100), (62), (46), (25), (2), (2), (1), (1), (1)
Host Mouse
(56), (50)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PAG1 antibody is un-conjugated
(3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(69), (55), (47), (33), (24), (13), (7), (6), (3), (1), (1)
Pubmed 5 references available
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Product Details anti-PAG1 Antibody

Target Details PAG1 Application Details Handling References for anti-PAG1 antibody (ABIN94270) Images
Specificity The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Cross-Reactivity (Details) Mouse, Human, Rat, Bovine, Other not tested
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Clone PAG-C1
Isotype IgG2b
Plasmids, Primers & others

Target Details PAG1

Product Details anti-PAG1 Antibody Application Details Handling References for anti-PAG1 antibody (ABIN94270) Images back to top
Antigen
Alternative Name PAG / Cbp (PAG1 Antibody Abstract)
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Pathways p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events

Application Details

Product Details anti-PAG1 Antibody Target Details PAG1 Handling References for anti-PAG1 antibody (ABIN94270) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details References for anti-PAG1 antibody (ABIN94270) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-PAG1 antibody (ABIN94270)

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling Images back to top
Background publications

Simeoni, Smida, Posevitz, Schraven, Lindquist: "Right time, right place: the organization of membrane proximal signaling." in: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004

Baumgartner, Angelisová, Setterblad, Mooney, Werling, Horejsí, Langsley: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." in: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003

Product cited in:

Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003

Vang, Abrahamsen, Myklebust, Horejsí, Taskén: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003

Images

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling References for anti-PAG1 antibody (ABIN94270) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody (ABIN94270) Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma c...
Immunohistochemistry (IHC) image for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody (ABIN94270) Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of ly...