anti-Human RFC2 antibody for Western Blotting

Recommended RFC2 Antibody (supplied by: Log in to see )

Antigen
Replication Factor C (Activator 1) 2, 40kDa (RFC2) Antibodies
  • 2610008M13Rik
  • 40kDa
  • AI326953
  • Recc2
  • RFC40
  • replication factor C subunit 2
  • replication factor C (activator 1) 2
  • LOC100231971
  • RFC2
  • Rfc2
Epitope
Center
15
14
7
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
84
40
38
7
4
4
4
3
3
3
1
1
Host
Rabbit
56
26
2
Clonality
Polyclonal
Conjugate
This RFC2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
69
22
18
18
13
13
8
3
1
Options
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Catalog No. ABIN2856035
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19 ABIN1490617 FACS IHC IHC (p) WB Mouse IgG1 AA 1-234 Log in to see 2B2 0
19 ABIN1490616 FACS IF IHC IHC (p) WB Mouse IgG1 AA 1-234 Log in to see 3A7 0
16.802607 ABIN519742 IF IHC (p) WB Mouse AA 1-320, full length Log in to see Polyclonal 0
16 ABIN1490618 FACS IHC IHC (p) WB Mouse IgG1 AA 1-234 Log in to see 4C11 0
16 ABIN1490619 FACS IHC IHC (p) WB Mouse IgG1 AA 1-234 Log in to see 5A1 0
13.802607 ABIN389297 FACS IHC (p) WB Rabbit Ig Fraction AA 17-46, N-Term Log in to see Polyclonal 1
13.802607 ABIN519740 IF WB Mouse AA 1-320, full length Log in to see Polyclonal 0
12.755764 ABIN3186738 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
12.755764 ABIN5620681 WB Rabbit Center Log in to see Polyclonal 0
12.755764 ABIN6146879 WB Rabbit Log in to see Polyclonal 0
10.802607 ABIN5537451 FACS IHC (p) WB Rabbit Ig Fraction AA 17-46, N-Term Log in to see Polyclonal 0
10.802607 ABIN3001633 FACS IHC WB Rabbit IgG Log in to see Polyclonal 0
7.8026066 ABIN2730770 IHC WB Mouse IgG1 Log in to see 3F6 0
7.8026066 ABIN1386502 IF (p) IHC (p) WB Rabbit IgG AA 325-354 Log in to see Polyclonal 0
7.8026066 ABIN562633 ELISA WB Mouse AA 245-353, partial Log in to see Polyclonal 0
7 ABIN1500671 FACS WB Mouse IgG1 Log in to see 11C6 0
7 ABIN1500672 IHC IHC (p) WB Mouse IgG1 Log in to see 3F6 0
4.8026066 ABIN2788844 WB Rabbit N-Term Log in to see Polyclonal 0
4.8026066 ABIN2788843 WB Rabbit Middle Region Log in to see Polyclonal 0
4.8026066 ABIN2706941 WB Rabbit Center Log in to see Polyclonal 0

General

Antigen Replication Factor C (Activator 1) 2, 40kDa (RFC2) Antibodies
Epitope Center
(15), (14), (7), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(84), (40), (38), (7), (4), (4), (4), (3), (3), (3), (1), (1)
Host Rabbit
(56), (26), (2)
Clonality
Polyclonal
Conjugate This RFC2 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(69), (22), (18), (18), (13), (13), (8), (3), (1)
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Product Details anti-RFC2 Antibody

Target Details RFC2 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to RFC2 (replication factor C (activator 1) 2, 40 kDa)
RFC2 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human RFC2. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details RFC2

Product Details anti-RFC2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RFC2 (RFC2 Antibody Abstract)
Background The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Cellular Localization: Nucleus
Molecular Weight 39 kDa
Gene ID 5982
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA

Application Details

Product Details anti-RFC2 Antibody Target Details RFC2 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , H1299

Restrictions For Research Use only

Handling

Product Details anti-RFC2 Antibody Target Details RFC2 Application Details Images back to top
Format Liquid
Concentration 0.43 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-RFC2 Antibody Target Details RFC2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Replication Factor C (Activator 1) 2, 40kDa (RFC2) (Center) antibody (ABIN2856035) WB Image RFC2 antibody detects RFC2 protein by western blot analysis. Various whole c...
Immunofluorescence (IF) image for anti-Replication Factor C (Activator 1) 2, 40kDa (RFC2) (Center) antibody (ABIN2856035) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RFC2, ...
Western Blotting (WB) image for anti-Replication Factor C (Activator 1) 2, 40kDa (RFC2) (Center) antibody (ABIN2856035) WB Image Sample (30 ug of whole cell lysate) A: A431 , B: H1299 C: Hela 10% SDS PAGE ...