anti-Rat (Rattus) RFC2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended RFC2 Antibody (supplied by: Log in to see )

Antigen
Replication Factor C (Activator 1) 2, 40kDa (RFC2) Antibodies
  • 2610008M13Rik
  • 40kDa
  • AI326953
  • Recc2
  • RFC40
  • replication factor C subunit 2
  • replication factor C (activator 1) 2
  • LOC100231971
  • RFC2
  • Rfc2
Epitope
AA 17-46, N-Term
15
14
6
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
84
39
38
7
4
4
4
3
3
3
1
1
Host
Rabbit
56
26
2
Clonality
Polyclonal
Conjugate
This RFC2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
69
21
18
18
13
12
9
4
1
Options
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Catalog No. ABIN389297
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.117413 ABIN1386502 IF (p) IHC (p) WB Rabbit IgG AA 325-354 Log in to see Polyclonal 0
1 ABIN1408376 IHC (p) WB HRP Rabbit IgG AA 325-354 Log in to see Polyclonal 0
1 ABIN1390483 IHC (p) WB Biotin Rabbit IgG AA 325-354 Log in to see Polyclonal 0

General

Antigen Replication Factor C (Activator 1) 2, 40kDa (RFC2) Antibodies
Epitope AA 17-46, N-Term
(15), (14), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(84), (39), (38), (7), (4), (4), (4), (3), (3), (3), (1), (1)
Host Rabbit
(56), (26), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This RFC2 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(69), (21), (18), (18), (13), (12), (9), (4), (1)
Pubmed 1 reference available
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Product Details anti-RFC2 Antibody

Target Details RFC2 Application Details Handling References for anti-RFC2 antibody (ABIN389297) Images
Specificity This RFC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24~54 amino acids from the N-terminal region of human RFC2.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This RFC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 AA from the N-terminal region of human RFC2.
Clone RB17303
Isotype Ig Fraction
Plasmids, Primers & others

Target Details RFC2

Product Details anti-RFC2 Antibody Application Details Handling References for anti-RFC2 antibody (ABIN389297) Images back to top
Antigen
Alternative Name RFC2 (RFC2 Antibody Abstract)
Background The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. RFC2 is the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of RFC2 gene has been associated with Williams syndrome.
Synonyms: Replication factor C subunit 2, Activator 1 subunit 2, Replication factor C 40 kDa subunit, Activator 1 40 kDa subunit, RFC2
Molecular Weight 39157 DA
Gene ID 5982
UniProt P35250
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA

Application Details

Product Details anti-RFC2 Antibody Target Details RFC2 Handling References for anti-RFC2 antibody (ABIN389297) Images back to top
Application Notes IHC (p) = 1:50-100, WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-RFC2 Antibody Target Details RFC2 Application Details References for anti-RFC2 antibody (ABIN389297) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-RFC2 antibody (ABIN389297)

Product Details anti-RFC2 Antibody Target Details RFC2 Application Details Handling Images back to top
Background publications

Tomida, Masuda, Hiroaki, Ishikawa, Song, Tsurimoto, Tateishi, Shiomi, Kamei, Kim, Kamiya, Vaziri, Ohmori, Todo: "DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex." in: The Journal of biological chemistry, Vol. 283, Issue 14, pp. 9071-9, 2008

Images

Product Details anti-RFC2 Antibody Target Details RFC2 Application Details Handling References for anti-RFC2 antibody (ABIN389297) back to top
Supplier Images
Western Blotting (WB) image for anti-Replication Factor C (Activator 1) 2, 40kDa (RFC2) (AA 17-46), (N-Term) antibody (ABIN389297) Western blot analysis of anti-RFC2 Antibody (N-term)(ABIN389297) in K562 cell line ly...
Immunohistochemistry (IHC) image for anti-Replication Factor C (Activator 1) 2, 40kDa (RFC2) (AA 17-46), (N-Term) antibody (ABIN389297) Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term)(...
Flow Cytometry (FACS) image for anti-Replication Factor C (Activator 1) 2, 40kDa (RFC2) (AA 17-46), (N-Term) antibody (ABIN389297) RFC2 Antibody (N-term) (ABIN389297) flow cytometry analysis of K562 cells (bottom his...
Western Blotting (WB) image for anti-Replication Factor C (Activator 1) 2, 40kDa (RFC2) (AA 17-46), (N-Term) antibody (ABIN389297) All lanes : Anti-RFC2 Antibody (N-term) at 1:1000 dilution Lane 1: K562 whole cell ly...