anti-Fungi (Fusarium) GRP78 antibody for Immunoprecipitation

Recommended GRP78 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) Antibodies
  • GRP78
  • BiP
  • GRP-78
  • grp78
  • hspa5a
  • BIP
  • MIF2
  • AL022860
  • AU019543
  • Bip
  • D2Wsu141e
  • D2Wsu17e
  • Grp78
  • Hsce70
  • SEZ-7
  • Sez7
  • baffled
  • mBiP
  • cb865
  • fb60h09
  • fi36d04
  • wu:fb60h09
  • wu:fi36d04
  • zgc:55994
  • zgc:77606
  • 78 kDa glucose-regulated protein
  • heat shock protein family A (Hsp70) member 5
  • BiP/GRP78
  • glucose-regulated protein 78
  • putative glucose-regulated protein 78
  • Hsp70 family ATPase KAR2
  • heat shock protein family A (Hsp70) member 5 S homeolog
  • heat shock 70 kDa protein 5a
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • heat shock protein 5
  • heat shock protein family A member 5
  • CpipJ_CPIJ003550
  • HSPA5
  • grp78
  • LOC100533358
  • BiP/grp78
  • Tc00.1047053506585.40
  • Tb11.02.5450
  • Tb11.02.5500
  • LMJF_28_1200
  • KAR2
  • LOC100135840
  • hspa5.S
  • hspa5
  • Hspa5
Reactivity
Cow (Bovine), Dog (Canine), Fungi (Fusarium), Hamster, Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis
678
483
456
121
118
116
114
92
60
57
57
56
53
36
23
22
8
4
3
3
2
1
1
1
1
Host
Rabbit
374
316
8
7
2
1
Clonality
Polyclonal
Conjugate
This GRP78 antibody is conjugated to Atto 680
42
37
31
25
24
22
20
19
18
18
18
18
18
18
18
17
14
9
9
8
8
8
8
8
8
6
6
6
5
5
4
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
660
319
283
264
205
142
62
49
44
14
13
11
7
6
3
3
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2486754
$ 496.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2486757 ICC IF IHC IP WB APC Rabbit Log in to see Polyclonal 7
1 ABIN2486748 ICC IF IHC IP WB Atto 390 Rabbit Log in to see Polyclonal 7
1 ABIN2486749 ICC IF IHC IP WB Atto 488 Rabbit Log in to see Polyclonal 7
1 ABIN2486751 ICC IF IHC IP WB Atto 594 Rabbit Log in to see Polyclonal 7
1 ABIN2486762 ICC IF IHC IP WB PerCP Rabbit Log in to see Polyclonal 7
1 ABIN2486764 ICC IF IHC IP WB Streptavidin Rabbit Log in to see Polyclonal 7
1 ABIN2486761 ICC IF IHC IP WB PE-Atto 594 Rabbit Log in to see Polyclonal 7
1 ABIN2486763 ICC IF IHC IP WB PE Rabbit Log in to see Polyclonal 7
1 ABIN2486753 ICC IF IHC IP WB Atto 655 Rabbit Log in to see Polyclonal 7
1 ABIN2486760 ICC IF IHC IP WB HRP Rabbit Log in to see Polyclonal 7
1 ABIN2486758 ICC IF IHC IP WB Biotin Rabbit Log in to see Polyclonal 7
1 ABIN2486750 ICC IF IHC IP WB Atto 565 Rabbit Log in to see Polyclonal 7
1 ABIN2486752 ICC IF IHC IP WB Atto 633 Rabbit Log in to see Polyclonal 7
1 ABIN2486755 ICC IF IHC IP WB Atto 700 Rabbit Log in to see Polyclonal 7
1 ABIN2486756 ICC IF IHC IP WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 7
1 ABIN2486759 ICC IF IHC IP WB FITC Rabbit Log in to see Polyclonal 7
1 ABIN361825 ICC IF IHC IP WB Rabbit Log in to see Polyclonal 10

General

Antigen Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Fungi (Fusarium), Hamster, Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis
(678), (483), (456), (121), (118), (116), (114), (92), (60), (57), (57), (56), (53), (36), (23), (22), (8), (4), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(374), (316), (8), (7), (2), (1)
Clonality
Polyclonal
Conjugate This GRP78 antibody is conjugated to Atto 680
(42), (37), (31), (25), (24), (22), (20), (19), (18), (18), (18), (18), (18), (18), (18), (17), (14), (9), (9), (8), (8), (8), (8), (8), (8), (6), (6), (6), (5), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(660), (319), (283), (264), (205), (142), (62), (49), (44), (14), (13), (11), (7), (6), (3), (3), (2)
Pubmed 7 references available
Supplier Log in to see

Product Details anti-GRP78 Antibody

Target Details GRP78 Application Details Handling References for anti-GRP78 antibody (ABIN2486754) Images
Specificity Detects ~78 kDa.
Purification Peptide Affinity Purified
Immunogen Rat GRP78 (Bip) synthetic peptide conjugated to KLH
Plasmids, Primers & others

Target Details GRP78

Product Details anti-GRP78 Antibody Application Details Handling References for anti-GRP78 antibody (ABIN2486754) Images back to top
Antigen
Alternative Name GRP78 (HSPA5 Antibody Abstract)
Background GRP78 is a ubiquitously expressed, 78- kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
Gene ID 25617
NCBI Accession NP_037215
UniProt P06761
Pathways Thyroid Hormone Synthesis, ER-Nucleus Signaling

Application Details

Product Details anti-GRP78 Antibody Target Details GRP78 Handling References for anti-GRP78 antibody (ABIN2486754) Images back to top
Application Notes
  • WB (1:1000)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

A 1:1000 dilution of SPC-107 was sufficient for detection of Grp78 in 10 μg of rat tissue lysate by ECL immunoblot analysis.

Restrictions For Research Use only

Handling

Product Details anti-GRP78 Antibody Target Details GRP78 Application Details References for anti-GRP78 antibody (ABIN2486754) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Storage -20 °C

References for anti-GRP78 antibody (ABIN2486754)

Product Details anti-GRP78 Antibody Target Details GRP78 Application Details Handling Images back to top
Background publications

Cho, Park, Kim, Kim, Kim, Jang: "BiP internal ribosomal entry site activity is controlled by heat-induced interaction of NSAP1." in: Molecular and cellular biology, Vol. 27, Issue 1, pp. 368-83, 2006

Luo, Mao, Lee, Lee: "GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development." in: Molecular and cellular biology, Vol. 26, Issue 15, pp. 5688-97, 2006

Dong, Ko, Baumeister, Swenson, Costa, Markland, Stiles, Patterson, Bates, Lee: "Vascular targeting and antiangiogenesis agents induce drug resistance effector GRP78 within the tumor microenvironment." in: Cancer research, Vol. 65, Issue 13, pp. 5785-91, 2005

Koomägi, Mattern, Volm: "Glucose-related protein (GRP78) and its relationship to the drug-resistance proteins P170, GST-pi, LRP56 and angiogenesis in non-small cell lung carcinomas." in: Anticancer research, Vol. 19, Issue 5B, pp. 4333-6, 2000

Yu, Luo, Fu, Mattson: "The endoplasmic reticulum stress-responsive protein GRP78 protects neurons against excitotoxicity and apoptosis: suppression of oxidative stress and stabilization of calcium homeostasis." in: Experimental neurology, Vol. 155, Issue 2, pp. 302-14, 1999

Laquerre, Anderson, Argnani, Glorioso: "Herpes simplex virus type 1 glycoprotein B requires a cysteine residue at position 633 for folding, processing, and incorporation into mature infectious virus particles." in: Journal of virology, Vol. 72, Issue 6, pp. 4940-9, 1998

Yang, Turner, Gaut: "The chaperone BiP/GRP78 binds to amyloid precursor protein and decreases Abeta40 and Abeta42 secretion." in: The Journal of biological chemistry, Vol. 273, Issue 40, pp. 25552-5, 1998

Images

Product Details anti-GRP78 Antibody Target Details GRP78 Application Details Handling References for anti-GRP78 antibody (ABIN2486754) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (Atto 680) (ABIN2486754) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal An...
Western Blotting (WB) image for anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (Atto 680) (ABIN2486754) Western blot analysis of Human, Dog, Mouse Cell line lysates showing detection of GRP...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (Atto 680) (ABIN2486754) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal An...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (Atto 680) (ABIN2486754) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal An...
Western Blotting (WB) image for anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (Atto 680) (ABIN2486754) Western blot analysis of Rat Tissue lysates showing detection of GRP78 protein using ...