GTPase NRas antibody (AA 167-179)
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- Target See all GTPase NRas (NRAS) Antibodies
- GTPase NRas (NRAS)
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Binding Specificity
- AA 167-179
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Reactivity
- Human, Mouse, Dog, Cow, Horse, Bat, Pig
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This GTPase NRas antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human NRAS.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%) Marmoset, Rat, Hamster, Guinea pig (92%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-RMKKLNSSDDGTQ from an internal region of human NRAS (NP_002515.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%), Marmoset, Rat, Hamster, Guinea pig (92%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NRAS Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.01 - 0.03 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- GTPase NRas (NRAS)
- Alternative Name
- NRAS / N-ras (NRAS Products)
- Synonyms
- NRAS antibody, AV095280 antibody, N-ras antibody, ALPS4 antibody, NRAS1 antibody, NS6 antibody, N-RAS antibody, fj99a10 antibody, wu:fj99a10 antibody, zgc:100761 antibody, zras-b1 antibody, H-RAS antibody, HRAS antibody, GTPase NRas antibody, neuroblastoma RAS viral (v-ras) oncogene homolog L homeolog antibody, NRAS proto-oncogene, GTPase antibody, neuroblastoma ras oncogene antibody, neuroblastoma RAS viral oncogene homolog antibody, Tsp_01117 antibody, nras.L antibody, Nras antibody, NRAS antibody, nras antibody
- Background
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Name/Gene ID: NRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806
Synonyms: NRAS, HRAS1, NRAS1, NS6, ALPS4, Transforming protein N-Ras, N-ras, N-ras protein part 4, GTPase NRas - Gene ID
- 4893
- NCBI Accession
- NP_002515
- UniProt
- P01111
- Pathways
- p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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