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|Antigen||Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies|
|Epitope||AA 593-606, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AIFM1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
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Product Details anti-AIFM1 AntibodyTarget Details AIFM1 Application Details Handling Images
|Specificity||This antibody detects AIF at C-term.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse, Rat|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Peptide corresponding to amino acids 593 to 606 of Human AIF .Remarks: This sequence is identical to those of Mouse and Rat AIF.|
Target Details AIFM1Product Details anti-AIFM1 Antibody Application Details Handling Images back to top
|Alternative Name||AIFM1 / AIF (AIFM1 Antibody Abstract)|
|Background||Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (2).Synonyms: Apoptosis-inducing factor 1 mitochondrial, PDCD8, Programmed cell death protein 8|
|Pathways||Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration|
Application DetailsProduct Details anti-AIFM1 Antibody Target Details AIFM1 Handling Images back to top
ELISA. Western blot: 0.25 to 1 μg/mL. A 67 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|