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|Application / Reactivity||Pig (Porcine)|
|Flow Cytometry (FACS)||25 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunofluorescence (IF)||3 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunoprecipitation (IP)||11 Antibodies|
|Western Blotting (WB)||1 Antibodies|
|Antigen||Integrin beta 2 (ITGB2) Antibodies|
|Reactivity||Pig (Porcine) Alternatives|
|Conjugate||This Integrin beta 2 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunoprecipitation (IP)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-Integrin beta 2 AntibodyTarget Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN180772) Images
|Specificity||This antibody recognises the porcine homologue of human CD18. Other species not tested.|
|Characteristics||Synonyms: Integrin beta-2, MFI7, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunitbeta, Complement receptor C3 subunit beta|
|Purification||Affinity chromatography on Protein G|
|Immunogen||Porcine large granular lymphocytes. Spleen cells from immunised Balb/c mice were fusedwith cells of the mouse P3-X63-Ag8. 653 myeloma cell line. Genename: CD18a|
Target Details Integrin beta 2Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN180772) Images back to top
|Alternative Name||CD18 / ITGB2 (ITGB2 Antibody Abstract)|
|Background||CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN180772) Images back to top
Immunoprecipitation. Clone PNK-I immunoprecipitates molecules of 166, 155 and 95 kDa under reducing andnon-reducing conditions. Flow Cytometry: use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN180772) Images back to top
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-Integrin beta 2 Antibody (ABIN180772)Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Dato, Kim: "Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 11, pp. 4452-62, 1990
ImagesProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN180772) back to top