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anti-Rat (Rattus) Integrin beta 2 antibody for Immunocytochemistry

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Epitope
Ser745
40
32
28
16
14
13
12
9
6
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
412
200
95
25
24
7
6
3
3
2
2
2
2
2
2
2
1
1
Host
Rabbit
282
165
153
5
2
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
90
70
50
19
9
7
6
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
395
240
184
109
82
65
61
44
39
34
16
9
7
6
4
3
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN189488
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN1859505 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 415-678 Log in to see Polyclonal
0.0010161072 ABIN2610761 ICC IHC (fro) FACS WB Biotin Mouse IgG1 Log in to see WT-3

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies 6 Antibodies
ELISA 14 Antibodies 19 Antibodies
Flow Cytometry (FACS) 27 Antibodies 123 Antibodies
Functional Studies (Func) 1 Antibodies 17 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 2 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Western Blotting (WB) 30 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Epitope Ser745
(40), (32), (28), (16), (14), (13), (12), (9), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(412), (200), (95), (25), (24), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(282), (165), (153), (5), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(90), (70), (50), (19), (9), (7), (6), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(395), (240), (184), (109), (82), (65), (61), (44), (39), (34), (16), (9), (7), (6), (4), (3), (2), (1), (1), (1), (1)
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling Images
Specificity The antibody only detects LFA-Beta2 phosphorylated on Serine 745.
Purification Tissue culture supernatant
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser745 of human LFA-Beta2 was used as immunogen.
Clone EP1288Y
Isotype IgG

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background In the immune system, integrins have essential roles in leukocyte trafficking andfunction. These include immune cell attachment to endothelial and antigen-presentingcells, cytotoxicity, and extravasation into tissues (1). Integrin adhesion receptorstransduce signals that control complex cell functions which require the regulation ofgene expression, such as proliferation, differentiation and survival. Their intracellulardomain has no catalytic function, indicating that interaction with other transducingmolecules is crucial for integrin-mediated signaling. JAB1 (Jun activationdomain-binding protein 1), a coactivator of the c-Jun transcription factor, has beenidentified as a protein that interacts with the cytoplasmic domain of the beta2 subunit ofthe alphaL/beta2 integrin LFA-1 (2). Patients with leukocyte adhesion molecule(CD11/CD18, beta 2 integrins) deficiency have structural defects in the common betasubunit (CD18), which prevent heterodimer formation and normal cell surfaceexpression of these receptors, leading to life-threatening bacterial infections (3). Alternate Names: anti-Beta 2 (antigen CD18 (p95) antibody, anti-CD 18 antibody, anti-CD18 antigenantibody, anti-Cell surface adhesion glycoproteins LFA 1/CR3/p150 95 subunit betaantibody, anti-Cell surface adhesion glycoproteins LFA 1/CR3/p15095 beta subunitantibody, anti-Complement component 3 receptor 3 and 4 subunit antibody,anti-Complement receptor C3 beta subunit antibody, anti-Integrin beta 2 antibody,anti-Integrin beta chain beta 2 antibody, anti-ITGB 2 antibody, anti-ITGB2 antibody,anti-LAD antibody, anti-LCAMB antibody, anti-Leukocyte Cell Adhesion Molecule CD18antibody, anti-LFA 1 antibody, anti-LFA1 antibody, anti-Lymphocyte function associatedantigen 1 antibody, anti-MAC 1 antibody, anti-MAC1 antibody, anti-Macrophage antigen1 (mac1) beta subunit antibody, anti-MF17 antibody, anti-MFI7 antibody.
Gene Symbol: ITGB2
Gene ID 3689, 16414, 309684
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 85kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250-500, Immunoprecipitation 1:100, Western Blot 1:1000-10000
Protocol Protocol specific for Integrin beta 2/CD18 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (Ser745) (ABIN189488) Western blot analysis on Jurkat cell lysates using anti-Phospho-LFA-?2 (pS745) RabMAb...
Immunohistochemistry (IHC) image for anti-Integrin beta 2 antibody (ITGB2) (Ser745) (ABIN189488) Immunohistochemical analysis of human tonsils using anti-Phospho- LFA-?2 (pS745) RabM...