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|Antigen||Integrin beta 2 (ITGB2) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This Integrin beta 2 antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-Integrin beta 2 AntibodyTarget Details Integrin beta 2 Application Details Handling Images
|Specificity||This antibody reacts with the beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. Clone WT.3 has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1. SM264A is recommended for this purpose.|
|Purification||Affinity chromatography on Protein G|
|Immunogen||FTL-43. Lymph node cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.|
Target Details Integrin beta 2Product Details anti-Integrin beta 2 Antibody Application Details Handling Images back to top
|Alternative Name||CD18 / ITGB2 (ITGB2 Antibody Abstract)|
|Background||CD18 is expressed by all leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation|
Application DetailsProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling Images back to top
Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Images back to top
|Buffer||PBS, pH 7.2 containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|