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|Application / Reactivity||Rat (Rattus)|
|ELISA||28 ELISA Kits|
|Antigen||Transforming Growth Factor, beta 1 (TGFB1) ELISA Kits|
|Reactivity||Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||15.6-1000 pg/mL|
|Minimum Detection Limit||15.6 pg/mL|
|16 references available|
|Supplier||Log in to see|
Product Details TGFB1 ELISA KitTarget details Application Details Handling References for TGFB1 Kit (ABIN416258) Images
|Purpose||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TGFb1 in Serum,Platelet-Poor Plasma,Tissue Homogenate,Cell Culture Supernatant,Biological Fluids|
|Sample Type||Serum, Platelet-Poor Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids|
|Specificity||This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1).|
|Cross-Reactivity (Details)||No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.|
|Material not included||
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|Alternative Name||TGFb1 (TGFB1 ELISA Kit Abstract)|
|Research Area||Growth Factors, Signaling|
|Pathways||TCR Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway|
Application DetailsProduct Details TGFB1 ELISA Kit Target details Handling References for TGFB1 Kit (ABIN416258) Images back to top
Information on standard material:
|Sample Volume||100 μL|
|Assay Time||3 h|
|Plate||Pre-coated,Strips (12 x 8)|
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGFb1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TGFb1.
Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
After TMB substrate solution is added, only those wells that contain TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of TGFb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, rinse tissues in ice-cold PBS (0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weigh before homogenization. Mince the tissues to small pieces and homogenize them in 5-10 mL of PBS with a glass homogenizer on ice (Micro Tissue Grinders work, too). Sonicate the resulting suspension with an ultrasonic cell disrupter or subject it to two freeze-thaw cycles to further break the cell membranes. Centrifugate the homogenates for 5 minutes at 5000 × g. Remove the supernate and assay immediately or aliquot and store at -20°C
Platelet-Poor Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g at 2-8°C within 30 minutes of collection. It is recommended to centrifuge samples for 10 minutes at 10,000 × g for complete platelet removal. Remove plasma and assay (see activation procedure) immediately or store samples in aliquot at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.
Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Calculation of Results||
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Construct a standard curve by plotting the mean O.D. and concentration for each standard and draw a best fit curve through the points on the graph or create a standard curve on log-log graph paper with TGFb1 concentration on the y-axis and absorbance on the x-axis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log of the data to establish standard curve for each test is recommended. Typical standard curve below is provided for reference only.
Intra-assay Precision (precision within an assay): 3 samples with low, middle and high level TGFb1 were tested 20 times on one plate, respectively.
Inter-assay Precision (precision between assays): 3 samples with low, middle and high level TGFb1 were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/mean X 100
|Restrictions||For Research Use only|
HandlingProduct Details TGFB1 ELISA Kit Target details Application Details References for TGFB1 Kit (ABIN416258) Images back to top
|Precaution of Use||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
|Handling Advice||To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.|
|Storage||4 °C/-20 °C|
|Expiry Date||6 months|
References for TGFB1 Kit (ABIN416258)Product Details TGFB1 ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Kabel, Elkhoely: "Ameliorative potential of fluoxetine/raloxifene combination on experimentally induced breast cancer." in: Tissue & cell, Vol. 48, Issue 2, pp. 89-95, 2016
Zhao, Zhang, Chai, Li, Cui, Wang, Meng, Liu, Wang, Li, Bai, Xiao: "Oxymatrine attenuates CCl4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathways." in: International immunopharmacology, Vol. 36, pp. 249-55, 2016
Bian, Sun: "Development of a KLD-12 polypeptide/TGF-?1-tissue scaffold promoting the differentiation of mesenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration." in: International journal of clinical and experimental pathology, Vol. 8, Issue 2, pp. 1093-103, 2015
Huang, Wang, Guo, Li, Yan, Yu, Wei, Li: "Effect of combined treatment with rosuvastatin and protein kinase C?2 inhibitor on angiogenesis following myocardial infarction in diabetic rats." in: International journal of molecular medicine, Vol. 35, Issue 3, pp. 829-38, 2015
Kabel, Abd Elmaaboud, Albarraq: "Ameliorative potential of omega 3 fatty acids and HMG-CoA reductase inhibitors on experimentally-induced non-alcoholic steatohepatitis." in: Prostaglandins, leukotrienes, and essential fatty acids, Vol. 96, pp. 1-9, 2015
Lemini, Ruiz-Herrera, Ledesma-Colunga, Díaz-Lezama, De Los Ríos, López-Barrera, Méndez, Martínez de la Escalera, Macotela, Clapp: "Prolactin anterior pituitary expression and circulating levels are reduced in obese and diabetic rats: role of TGF-? and TNF-?." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 308, Issue 9, pp. R792-9, 2015
Wang, Cao, Cui, Kong, Tian, Cai, Wu, Han, Zhao, Xia: "Salvia Miltiorrhiza Bge.f.alba Ameliorates the Progression of Monocrotaline-Induced Pulmonary Hypertension by Protecting Endothelial Injury in Rats." in: The Tohoku journal of experimental medicine, Vol. 236, Issue 2, pp. 155-62, 2015
Marshall-Webb, Fosh: "Gas-containing gallstones: a radiological finding." in: ANZ journal of surgery, 2015
Zhao, Shi, Li, Li, Zhao: "Silymarin attenuates paraquat-induced lung injury via Nrf2-mediated pathway in vivo and in vitro." in: Clinical and experimental pharmacology & physiology, 2015
Chen, Liu, Wang, Wu, Cong, You, Jia: "NIM811 downregulates transforming growth factor‑β signal transduction in vivo and in vitro." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 522-8, 2015
Maheshwari, Balaraman, Sen, Seth: "Effect of coenzyme Q10 alone and its combination with metformin on streptozotocin-nicotinamide-induced diabetic nephropathy in rats." in: Indian journal of pharmacology, Vol. 46, Issue 6, pp. 627-32, 2014
Bobowiec, Wojcik, Jaworska-Adamu, Tusinska: "Fibrogenic response of hepatic stellate cells in ovariectomised rats exposed to ketogenic diet." in: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, Vol. 64, Issue 1, pp. 19-26, 2013
Yang, Yuan, Chen, Xiong, Ruan: "Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats." in: Journal of ethnopharmacology, Vol. 151, Issue 1, pp. 343-51, 2013
Cao, Deng, Wei, Yang, Su, Wei, Xu, Yu et al.: "Incorporating pTGF-?1/calcium phosphate nanoparticles with fibronectin into 3-dimensional collagen/chitosan scaffolds: efficient, sustained gene delivery to stem cells for chondrogenic ..." in: European cells & materials, Vol. 23, pp. 81-93, 2012
Abramovich, Irusta, Bas, Cataldi, Parborell, Tesone: "Angiopoietins/TIE2 system and VEGF are involved in ovarian function in a DHEA rat model of polycystic ovary syndrome." in: Endocrinology, Vol. 153, Issue 7, pp. 3446-56, 2012
Miao, Guo, Wang, Cui, Yang, Huang, Qin, Chen, Li, Wang, Wei: "The intervention effect of Rosiglitozone in ovarian fibrosis of PCOS rats." in: Biomedical and environmental sciences : BES, Vol. 25, Issue 1, pp. 46-52, 2012
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