anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) Antibodies

Mouse (Murine) Neutrophil Cytosolic Factor 4, 40kDa (NCF4) interaction partners

1. These studies demonstrate a critical role of phosphatidylinositol 3-phosphate-p40(phox) binding for optimal activation of the NADPH oxidase in macrophages.

2. Our data reveal a specific role for NCF4-mediated intracellular ROS production in regulating autoimmunity and chronic inflammation.

3. p40phox deficiency leads to upregulation of chemokine receptor 1 and downregulation of fucosyltransferases and sialyltransferases involved in glycan modifications during experimental intestinal inflammation.

4. cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation during Fcgamma receptor (FcgammaR)-mediated phagocytosis

5. The defect in Reactive Oxygen Species production and killing of s aureus by p40(phox-/-) neutrophils defines p40(phox) as an essential component in bacterial killing.

6. p40(phoxR58A/R58A) embryos do not develop normally but p40(phoxR58A/-) mice are viable and neutrophils from these animals exhibit significantly reduced oxidase responses compared to those from their p40(phox+/-) siblings.

7. binding of the ternary complex to phagosomes can be temporally regulated by switching between adaptor proteins that have PX domains with distinct lipid-binding specificities

8. Class III PI3K Vps34 is responsible for the synthesis of PtdIns(3)P on phagosomes containing either S aureus or E coli. PtdIns(3)P binding to p40(phox) is important for CD18-dependent activation oxidase activation in response to S aureus and E coli

9. p40(phox) is prephosphorylated in resting endothelial cells and can compensate p47(phox) in keeping basal ROS production.

Human Neutrophil Cytosolic Factor 4, 40kDa (NCF4) interaction partners

1. We report on 24 p40phox-deficient patients from 12 additional families in 8 countries. These patients display 8 different in-frame or out-of-frame mutations of NCF4 that are homozygous in 11 of the families and compound heterozygous in another.

2. Tyrosine kinase substrate (Tks) proteins, analogous to the related proteins p47(phox), p40(phox) and NoxO1, also facilitate local generation of reactive oxygen species (ROS), which aid in signaling at invadopodia and/or podosomes to promote their activity. As their name suggests, Tks adaptor proteins are substrates for tyrosine kinases, especially Src. [review]

3. Phosphoinositol 3-phosphate regulates reactive oxygen species production by maintaining p40phox and p67phox at the phagosomal membrane.

4. NCF4 polymorphism was associated with Crohn's disease, but not ulcerative colitis in Caucasian--{REVIEW}

5. NCF4 may induce ex- pression of NADPH oxidase enzymes, such as p67phox, p47phox, p22phox and NOX2, which lead to increased ROS levels.

6. Germline variation in NCF4, an innate immunity gene, is associated with an increased risk of colorectal cancer.

7. The contribution of the functionally relevant NADPH polymorphisms rs1883112 and rs4673 to anthracycline-related heart lesions provides a plausible explanation for their modulation of cardiotoxicity.

8. Constitutive and inducible intracellular production of reactive oxygen species (ROS) is higher in B cells expressing functional p40phox, supporting a direct role for p40phox in regulating B cell intracellular ROS generation.

9. Genome-wide association studies-reported associations between the NELL1, NCF4, and FAM92B genes and susceptibility to Crohn's disease could not be replicated in Canadian children and young adults.

10. Younger age at diagnosis, complicated disease behavior, and ileal disease location are risk factors for perianal CD. First report of an association of the NCF4 gene with perianal disease.

11. cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation during Fcgamma receptor (FcgammaR)-mediated phagocytosis

12. Results demonstrate that PBEF can prime for PMN respiratory burst activity by promoting p40 and p47 translocation to the membrane.

13. no association between SNP rs4821544 and the presence of granulomas in Crohn's disease

14. cytosolic localisation depends on direct interaction with F-actin

15. All mutations and some polymorphisms identified in the NCF4 gene in the autosomal forms of chronic granulomatous disease are listed. Review.

16. p40(phox) functions primarily to regulate Fc gamma receptor-induced NADPH oxidase activity rather than assembly, and stimulates superoxide production via a phosphatidylinositol-3-phosphate signal that increases after phagosome internalization.

17. detailed study of the protein-protein interactions that occur in the p40-p47-p67(phox) complex of the resting oxidase

18. p22(phox), gp91(phox), p47(phox), p67(phox), and p40(phox) existed as a functional complex in the cytoskeletal fraction.

19. Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils

20. Multiple PU.1 sites cooperate in the regulation of p40(phox) transcription during granulocytic differentiation of myeloid cells.

Cow (Bovine) Neutrophil Cytosolic Factor 4, 40kDa (NCF4) interaction partners

1. NCF4 expression was regulated by the miRNA-mRNA interaction mechanism, and an important role for NCF4 in mastitis susceptibility in dairy cow was suggested.

2. Study proposes that NCF4 splice variants generated by functional SNP are important risk factors for mastitis susceptibility in dairy cows.