anti-Chicken Heat Shock 60kDa Protein 1 (Chaperonin) antibody for ELISA

Recommended Heat Shock 60kDa Protein 1 (Chaperonin) Antibody (supplied by: Log in to see )

Antigen
Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antibodies
  • CPN60
  • GROEL
  • HLD4
  • HSP-60
  • HSP60
  • HSP65
  • HuCHA60
  • SPG13
  • chaperonin
  • cpn60
  • groel
  • hld4
  • hsp60
  • hsp65
  • spg13
  • 60kDa
  • Hsp60
  • 12
  • BP5
  • CG12101
  • Cpn60
  • Dmel\\CG12101
  • Dmhsp60
  • G62
  • HSP60A
  • Hsp60A
  • IEF16
  • Mmp-P1
  • SSP 7506
  • d-hsp60
  • hsp60A
  • l(1)10Ac
  • l(1)BP5
  • l(1)G8
  • l(1)HM21
  • l(1)L12
  • l(1)dp025
  • cb863
  • fa04a05
  • fb22d10
  • fi27b05
  • id:ibd2197
  • sb:cb144
  • wu:fa04a05
  • wu:fb22d10
  • wu:fi04a12
  • wu:fi27b05
  • MIF4
  • MNA2
  • mopA
  • groL
  • crpA
  • Hspd1-30p
  • heat shock protein family D (Hsp60) member 1
  • heat shock protein family D (Hsp60) member 1 S homeolog
  • 60 kDa heat shock protein, mitochondrial
  • heat shock protein 1 (chaperonin)
  • Heat shock protein 60A
  • heat shock 60 protein 1
  • chaperone ATPase HSP60
  • molecular chaperone GroEL
  • thermosome subunit
  • chaperonin GroEL
  • mitochondrial chaperonin
  • heat shock protein family D member 1
  • HSPD1
  • hspd1.S
  • hspd1
  • LOC100414401
  • Hspd1
  • Hsp60A
  • HSP60
  • groEL
  • MMP_RS07785
  • groEl
  • LOC100136430
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Plant, Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
809
565
549
386
341
324
315
302
278
268
190
179
132
120
110
105
77
60
40
38
37
37
36
36
23
21
20
20
18
18
18
17
17
6
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
Host
Mouse
540
344
10
Clonality
Monoclonal
Conjugate
Atto 488
33
25
24
24
21
21
17
12
12
12
12
12
12
12
12
11
11
10
10
10
10
10
9
9
8
8
8
8
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
749
415
401
355
294
247
227
161
85
63
42
41
8
7
6
6
2
2
1
1
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Catalog No. ABIN2481429
$ 486.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.755764 ABIN2481431 FACS IP IHC ELISA WB Atto 594 Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481435 FACS IP IHC ELISA WB Atto 700 Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481436 FACS IP IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481438 FACS IP IHC ELISA WB Biotin Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481442 FACS IP IHC ELISA WB PerCP Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481443 FACS IP IHC ELISA WB PE Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481444 FACS IP IHC ELISA WB Streptavidin Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481441 FACS IP IHC ELISA WB PE-Atto 594 Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481433 FACS IP IHC ELISA WB Atto 655 Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481434 FACS IP IHC ELISA WB Atto 680 Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481430 FACS IP IHC ELISA WB Atto 565 Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481439 FACS IP IHC ELISA WB FITC Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481437 FACS IP IHC ELISA WB APC Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481432 FACS IP IHC ELISA WB Atto 633 Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481440 FACS IP IHC ELISA WB HRP Mouse IgG1 Log in to see LK1 2
27.755764 ABIN2481428 FACS IP IHC ELISA WB Atto 390 Mouse IgG1 Log in to see LK1 2
21.755764 ABIN2486148 IP IHC ELISA WB Atto 390 Rabbit Log in to see Polyclonal 12
21.755764 ABIN2486150 IP IHC ELISA WB Atto 565 Rabbit Log in to see Polyclonal 12
21.755764 ABIN2486151 IP IHC ELISA WB Atto 594 Rabbit Log in to see Polyclonal 12
21.755764 ABIN2486152 IP IHC ELISA WB Atto 633 Rabbit Log in to see Polyclonal 12

General

Antigen Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antibodies
Reactivity Chicken, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Plant, Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
(809), (565), (549), (386), (341), (324), (315), (302), (278), (268), (190), (179), (132), (120), (110), (105), (77), (60), (40), (38), (37), (37), (36), (36), (23), (21), (20), (20), (18), (18), (18), (17), (17), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(540), (344), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate Atto 488
(33), (25), (24), (24), (21), (21), (17), (12), (12), (12), (12), (12), (12), (12), (12), (11), (11), (10), (10), (10), (10), (10), (9), (9), (8), (8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(749), (415), (401), (355), (294), (247), (227), (161), (85), (63), (42), (41), (8), (7), (6), (6), (2), (2), (1), (1)
Pubmed 2 references available
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Product details

Target Details Application Details Handling References Images
Specificity Detects ~60 kDa.
Purification Protein G Purified
Immunogen Recombinant human HSP60
Clone LK1
Isotype IgG1

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name HSP60 (HSPD1 Antibody Abstract)
Background In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
Gene ID 3329
NCBI Accession NP_002147
UniProt P10809
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product details Target Details Handling References Images back to top
Application Notes
  • WB (1:20000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • IP (1:200)
  • optimal dilutions for assays should be determined by the user.
Comment

0.05 μg/ml of SMC-110 was sufficient for detection of HSP60 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References

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Background publications

Bukau, Horwich: "The Hsp70 and Hsp60 chaperone machines." in: Cell, Vol. 92, Issue 3, pp. 351-66, 1998

Hartl: "Molecular chaperones in cellular protein folding." in: Nature, Vol. 381, Issue 6583, pp. 571-9, 1996

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (Atto 488) (ABIN2481429) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Ant...
Immunohistochemistry (IHC) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (Atto 488) (ABIN2481429) Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 ...
Western Blotting (WB) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (Atto 488) (ABIN2481429) Western Blot analysis of Human Cell line lysates showing detection of Hsp60 protein u...
Immunohistochemistry (IHC) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (Atto 488) (ABIN2481429) Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 ...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (Atto 488) (ABIN2481429) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Ant...