E-cadherin antibody (AA 784-870)

Catalog No. ABIN1077673

Product Details anti-E-cadherin Antibody

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Binding Specificity: AA 784-870

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to E-cadherin

Specificity: The antibody is a rabbit polyclonal antibody raised against E-cadherin. It has been selected for its ability to recognize E-cadherin in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant E-cadherin corresdonding to Pro784~Gly870 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-3 μg/mL

Immunohistochemistry: 5-30 μg/mL

Immunocytochemistry: 5-30 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: E-cadherin (CDH1 Products)

Synonyms: Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody

Background: CDHE, CD324, CDH1, Arc1, ECAD, CDH-E, E-CAD, LCAM, UVO, Cadherin 1 Type 1, Uvomorulin, E-Cadherin, Calcium-Dependent Adhesion Protein, Epithelial, Liver Cell Adhesion Molecule

Molecular Weight: 28.3 kDa

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation