BDNF antibody

Catalog No. ABIN1100212

Product Details anti-BDNF Antibody

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody is specific for human BDNF protein. A BLAST analysis was used to suggest cross-reactivity with BDNF from human sources based on 100 % homology with the immunizing sequence. Based on high to 100 % homology, there is a chance of cross-reactivity to BDNF from a wide variety of animals. Cross-reactivity with BDNF from other sources has not been determined.

Purification: Ion exchange chromatography

Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated human BDNF protein produced in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:1000)

Usage: This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kD in size corresponding to the mature human BDNF protein by western blotting in appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF (BDNF Products)

Synonyms: ANON2 antibody, BULN2 antibody, bdnf-A antibody, BDNF antibody, bdnf antibody, brain derived neurotrophic factor antibody, brain-derived neurotrophic factor antibody, brain-derived neurotrophic factor L homeolog antibody, brain-derived neurotrophic factor S homeolog antibody, BDNF antibody, Bdnf antibody, bdnf antibody, bdnf.L antibody, bdnf.S antibody

Background: Name/Gene ID: BDNF
Family: NGF Beta

Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin

Gene ID: 627

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity