FAK antibody (AA 706-1052)

Catalog No. ABIN1107179

Product Details anti-FAK Antibody

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Binding Specificity: AA 706-1052

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: This antibody detects human, mouse and rat FAK in Western blotting, Immunoprecipitation and immunocytostaining. This antibody detects ~125 kDa of human FAK on Westernblotting with whole cell lysates from human cell line such as HeLa. Since active FAK is highly phosphorylated, position of FAK band on westernblotting may varry with its phosphorylation level.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Protein-A Sepharose

Immunogen: GST fusion protein containing human FAK (residues 706-1052 a.a.)

Clone: 12G4

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 50 % glycerol, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: FAK1 / PTK2 (PTK2 Products)

Synonyms: FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, PTK2 antibody, Fadk antibody, mKIAA4203 antibody, fak antibody, fak1a antibody, ptk2 antibody, ptk2a antibody, wu:fa18c04 antibody, zgc:66114 antibody, CG10023 antibody, CT28129 antibody, DFAK antibody, DFak antibody, DFak56 antibody, Dfak antibody, Dfak56 antibody, DmFAK antibody, Dmel\\CG10023 antibody, Fak56 antibody, Fak56D antibody, fak56 antibody, protein tyrosine kinase 2 antibody, protein tyrosine kinase 2 L homeolog antibody, PTK2 protein tyrosine kinase 2 antibody, protein tyrosine kinase 2ab antibody, Focal adhesion kinase antibody, PTK2 antibody, ptk2.L antibody, Ptk2 antibody, ptk2ab antibody, Fak antibody

Background: FAK (focal adhesion kinase) is a protein tyrosine kinase which colocalizes with integrin receptors at cell-substratum contact sites (focal adhesions). It is thought that enhanced tyrosine phosphorylation of FAK is important for integrin mediated signaling as well as for other signalings. FAK associates with a number of proteins such as Src, Shc, PI-3 kinase, Grb2, Cas, Cas-L, paxilin and transmamblane proteins, which enables FAK to couple to a variety of different intracellular transduction pathways in adherent and non-adherent cells. FAK is involved in anchorage-dependent survival signal through PI-3 kinase and also may play a role in cell growth, migration, cytoskeletal organization.Synonyms: FADK1, FAK, Focal adhesion kinase 1, Protein-tyrosine kinase 2, pp125FAK

Gene ID: 5747

NCBI Accession: NP_722560

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling